Sequences from confirmation amplicon


Confirmation sequences for line 822A03 and hit At5g12350 are shown.
Line ID 822A03
Locus At5g12350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3999130
Primers used for PCR 41ny - 8474, show primer details
   
Sequence Name 12-K114782-0022-822-A03C-41ny
GenBank Accession LN491690 [GenBank]
Sequencing primer gene specific primer, 41ny: 5'-TTAACGACCTCTTGGCTTAGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114782-0022-822-A03C-41ny
AATTTTGGTGTGGCTAGTCCTGAAGAGTGGGAGTCGGTGTAGTTGACCGGGGTGGACTAG
GACGCCTCGATATTGGGGAAGTTGCCCGAGACACAATTCGAGAACCAGGAACAGAAGCTG
AGAAGAACTTCTTTGATGCTCCAAATACTGGATTGAAAGACTTGGCTATGTTAAGTGCAC
CCCGTTGAGAGCTTCCACTCGGTATGGGTGAGACACGACTACTATTGAATTCATACTTCT
TGTTCTTCTTATGTCGACTGTCCACTTGTCTCATGGATTCCATTAATGAAAATCTAGCTA
ACTGTCCATCGGATCTAGAATCGAACTTGTCATCTTTGTCAATGGGATCTGATCCCTGGA
AAATACGGCAGCTGCCGCTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 12-K114769-0022-822-A03C-8409
GenBank Accession LN491689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114769-0022-822-A03C-8409
ACAGTTAGCTAGATTTTTCATTAATGGAATCCATGAGACAAGCGGACAGTCGACATAAGA
AGAACAAGAAGTATGAATTCAATAGTAGTCGTGTCTCACCCATACCGAGTGGAAGCTCTC
AACGGGGTGCACTTAACATAGCCAAGTCTTTCAATCCAGTATTTGGAGCATCAAAGAAGT
TCTTCTCAGCTTCTGTTCCTGGTTCTCGAATTGTGTCTCGGGCAACTTCCCCAATATCGA
GGCGTCCTAGTCCACCCCGGTCAACTACACCGACTCCCACTCTTTCAGGACTAGCCACAC
CAAAATTTGTAGTGGATGATACTAAGAGAACCAATGATAACCTAAGCCAAGGTTTTCT

Last Updated on 06.12.2018 16:11