Sequences from confirmation amplicon


Confirmation sequences for line 822A09 and hit At2g42090 are shown.
Line ID 822A09
Locus At2g42090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17556862
Primers used for PCR 08on - 8474, show primer details
   
Sequence Name 12-K114972-0022-822-A09C-08on
GenBank Accession LN491698 [GenBank]
Sequencing primer gene specific primer, 08on: 5'-GTGAGAATATCTCTGTTGGCATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114972-0022-822-A09C-08on
ATCCATCTCTTGGACGACCCCGACACATGGAAACACAACCTTTGGTGCTTCGTCTCCAGC
AAATCCCGCCTTCAAAATGTTTCACCAATTAATTGGCACTTAACAAAACGTTTCACGAGA
AATACTCCAGAGTATAGGTCAAAATATGGTTTATTTTAATAAAACAATCGAACACTGAAA
CAAAATGAAATGGAAAAAACTACAAATAAATACGGAGAAAATCAATATATTCCCCTTTCT
TTATTGGCGGCCGAGTGTTAGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 03-K114971-0022-822-A09C-8409
GenBank Accession LN487603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114971-0022-822-A09C-8409
CATTTCATTGAATATATCCTGCTCTAACACTCGGCCGCCAATAAAGAAAGGGGAATATAT
TGATTTTCTCCGTATTTATTTGTAGTTTTTTCCATTTCATTTTGTTTCAGTGTTCGATTG
TTTTATTAAAATAAACCATATTTTGACCTATACTCTGGAGTATTTCTCGTGAAACGTTTT
GTTAAGTGCCAATTAATTGGTGAAACATTTTGAAGGCGGGATTTGCTGGAGACGAAGCAC
CAAAGGTTGTGTTTCCATGTGTCGTGGGTCGTCCAAGAGATGGATTGAATCCGAACGAAT
CTTATGTGGGAGAAGAAGGTCATGCCAACAGAGATATTCTCA

Last Updated on 06.12.2018 16:11