Sequences from confirmation amplicon


Confirmation sequences for line 822B12 and hit At5g58300 are shown.
Line ID 822B12
Locus At5g58300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23574239
Primers used for PCR CS06 - 8474, show primer details
   
Sequence Name 20-K027845-022-822-B12C-8409
GenBank Accession LN495070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K027845-022-822-B12C-8409
GAGCCTTTCAATTGAATATATTTACTCTTTCTTTACCTCGTTGGTAACCGGGGAAGTGAG
AAAACGCCTCTTGACTGGGACTCCAGAGTAAAAATCACACTATCAGCGGCAAAAGGTATA
GCTCATCTCCATGCAGCTGGAGGTCCAAAGTTCAGTCATGGAAACATAAAGTCCTCAAAT
GTGATTATGAAGCAGGAGAGCGATGCGTGCATTTCTGATTTTGGACTGACCCCTCTGATG
GCTGTGCCGATTGCTCCAATGAGAGGTGCAGGCTACCGCGCACCAGAAGTAATGGAAACA
AGGAAACACACACATAAATCCGATGTTTACAGTTTCGGTGTACTTATACTAGAGATGTTA
ACTGGGAAGTCTCCAGTGCAATCACCAAGCCGGGATGACATGGTTGATCTCCCGAGGTGG
GTACAGTCAGTGGTGAGAGAGGAATGGACAAGCGAGGTGTTTGATATTGAGCTGATGAGG
TTTC
   
Sequence Name 21-K027845-022-822-B12C-CS06
GenBank Accession LN495525 [GenBank]
Sequencing primer gene specific primer, CS06: 5'-CTTCAATGTTCTGAAACCTCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027845-022-822-B12C-CS06
TGTCATTCCTCTCTCACCACTGACTGTACCCACCTCNGGGAGATCAACCATGTCATCCCG
GCTTGGTGATTGCACTGGAGACTTCCCAGTTAACATCTCTAGTATAAGTACACCGAAACT
GTAAACATCGGATTTATGTGTGTGTTTCCTTGTTTCCATTACTTCTGGTGCGCGGTAGCC
TGCACCTCTCATTGGAGCAATCGGCACAGCCATCAGAGGGGTCAGTCCAAAATCAGAAAT
GCACGCATCGCTCTCCTGCTTCATAATCACATTTGAGGACTTTATGTTTCCATGACTGAA
CTTTGGACCTCCAGCTGCATGGAGATGAGCTATACCTTTTGCCGCTGATAGTGTGATTTT
TACTCTGGAGTCCCAGTCAAGAGGCGTTTTCTCACTTCCCCGGTTACCAACGAGGTAAAG
AAAGAGTAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACTT

Last Updated on 06.12.2018 16:11