Sequences from confirmation amplicon


Confirmation sequences for line 823C07 and hit At4g36880 are shown.
Line ID 823C07
Locus At4g36880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17375010
Primers used for PCR 53nb - 8474, show primer details
   
Sequence Name 30-K114626-0022-823-C07C-53nb
GenBank Accession LN499830 [GenBank]
Sequencing primer gene specific primer, 53nb: 5'-TGTTTGTGTATTGATCAACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114626-0022-823-C07C-53nb
GTATAAACAAGATCGTAACAGGAGAACTCATATCTCTATCAGAACAAGAACTTGTTGACT
GCGACAAATCCTACAATCAAGGTTGCAACGGCGGTTTAATGGACTACGCTTTTCAATTCA
TCATGAAAAATGGTGGCTTAAACACTGAGAAAGATTATCCTTACCGTGGATTCGGCGGAA
AATGCAATTCTTTCTTGGTATACACTCAAAAACAGAGTTGCTCTGTTTTCACTTTATTTT
CAAGAATTGCAAAATCTGATTATTTTTCCTATCTCTTTGTGTTTTTGCAGAAGAATTCTA
GAGTTGTGAGTATTGATGGGTACGAAGATGTTCCTACTAAAGACGAGACTGCGTTGAAGA
AAGCTATTTCATACCAACCGGTTAGTGTAGCTATTGAAGCCGGTGGAAGAATTTTTCAAC
ATTACCAATCGGTAAGCGAACCGACATTGATTTTGACCCGGTTTAAACCCGGTTTTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTATTA
   
Sequence Name 30-K114625-0022-823-C07C-8409
GenBank Accession LN499829 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114625-0022-823-C07C-8409
TCATTGAATATATCCTGAAAACCGGGGTTAAACCGGGTCAAAATCAATGTCGGTTCGCTT
ACCGATTGGTAATGTTGAAAAATTCTTCCACCGGCTTCAATAGCTACACTAACCGGTTGG
TATGAAATAGCTTTCTTCAACGCAGTCTCGTCTTTAGTAGGAACATCTTCGTACCCATCA
ATACTCACAACTCTAGAATTCTTCTGCAAAAACACAAAGAGATAGGAAAAATAATCAGAT
TTTGCAATTCTTGAAAATAAAGTGAAAACAGAGCAACTCTGTTTTTGAGTGTATACCAAG
AAAGAATTGCATTTTCCGCCGAATCCACGGTAAGGATAATCTTTCTCAGTGTTTAAGCCA
CCATTTTTCATGATGAATTGAAAAGCGTAGTCCATTAAACCGCCGTTGCAACCTTGATTG
TAGGATTTGTCGCAGTCAACAAGTTCTTGTTCTGATAGAGATATGAGTTCTCCTGTTACG
ATCTTGTTTATACCTTCTACTGCTGCAGTAGTCGAAAACGCCCAACAACTTCCTGTGTTG
ATCATANCACAAAC

Last Updated on 06.12.2018 16:11