Sequences from confirmation amplicon


Confirmation sequences for line 825B03 and hit At2g42290 are shown.
Line ID 825B03
Locus At2g42290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17618950
Primers used for PCR 78ra - 8474, show primer details
   
Sequence Name 54-K116162-0022-825-B03C-78ra
GenBank Accession LN508590 [GenBank]
Sequencing primer gene specific primer, 78ra: 5'-TCAAACGCTACGAATTTACCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116162-0022-825-B03C-78ra
TCTTTGTCTCGAATTGTACCATCGGAGCTTCGTTTTCTCCGGATCAGCCATACAGAGAGA
GAAACAGCTCCGATTACAACCGAAACTCCAGAAATCAGAGAAACGGTTACTGAACCGGTG
ATCTGCTGCTTCTTCTCCTTTGCGTCGTCATTGCTGATTACACTAGGGTTTGGTTTCTGG
AGTTCTTGAGTACCTTCCGGCTTTGCAGCGACGAAATTTGGGGTTTTGATTTTTTCACAC
GGCGTTTGCAATGGGAAGCCACAGAGATGAGAGTTTCCAGCGAATGCGTTTGGTCCTTGA
TTCAACAGAGATCCCACCTGAGGTACTTTTCCGGTGAGATTGTTGTGACTAAAATCTAAG
CTGACGTGAACTCGAAATCGACCGTACGACGGTGGAATCTCGCCGGTGAATTGAGTGGAA
CTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 25-K116161-0022-825-B03C-8409
GenBank Accession LN497603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116161-0022-825-B03C-8409
ATTTCATTGAATATATCCTGAAGTTCCCTCAATTCACCGGCGAGATTCCACCGTCGTACG
GTCGATTTCGAGTTCACGTCAGCTTAGATTTTAGTCACAACAATCTCACCGGAAAAGTAC
CTCAGGTGGGATCTCTGTTGAATCAAGGACCAAACGCATTCGCTGGAAACTCTCATCTCT
GTGGCTTCCCATTGCAAACGCCGTGTGAAAAAATCAAAACCCCAAATTTCGTCGCTGCAA
AGCCGGAAGGTACTCAAGAACTCCAGAAACCAAACCCTAGTGTAATCAGCAATGACGACG
CAAAGGAGAAGAAGCAGCAGATCACCGGTTCAGTAACCGTTTCTCTGATTTCTGGAGTTT
CGGTTGTAATCGGAGCTGTTTCTCTCTCTGTATGGCTGATCCGGAGAAAACGAAGCTCCG
ATGGGTACAATTCGGAGACAAAGACGACGACGGTGGTTTCAGAGTTCGACGAAGAAGGGC
AAGAAGGTAAATTCTAAGCCGTTTGA

Last Updated on 06.12.2018 16:11