Sequences from confirmation amplicon


Confirmation sequences for line 827C11 and hit At4g39390 are shown.
Line ID 827C11
Locus At4g39390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18317766
Primers used for PCR BF04 - 8474, show primer details
   
Sequence Name 27-K028746-022-827-C11C-8409
GenBank Accession LN498246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028746-022-827-C11C-8409
CCCGGGTTTCGAAGAACGCATTAACCGTAACCAGAATTCTACCGTGTATAGCAATCAGCA
TTCCAAGCACTAACTCGTAGGTTTAATCCTTCCTTACCGAAGAAAGTGAATCCCAAAACC
AAAACCAGAATTCGTCTTCATGTGACCAAGTACTTGGAAAGACACCGCCGTGAATCTTCC
AATACAGATGAATTGGCTGAGATTGGTCCCAACCGCGATACTGCACGAGAGTATTAAGAA
GAACTGCCCAAAAAATTGTGTTAGATAGAGCTTAGGAAAGACAAATCCAACAGAGTATAA
CAATCAGTTCTTGAGATGGAATAAATGCGAATTGGTATGACTACTAATACTACACTATGT
TCCAAATACTCATTGCTCTGTTTTGAATCGTAGGAGACTTTGTAAAGAAACGGAGAAGAA
ACTTTACCAGAGATACAAATGAAAAGTTGTATGCATCCACTCTTTGGTTAGTCAACCAGT
AGTCTAG
   
Sequence Name 27-K028746-022-827-C11C-BF04
GenBank Accession LN498247 [GenBank]
Sequencing primer gene specific primer, BF04: 5'-GGTCGGACCATTTCTAGACTACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028746-022-827-C11C-BF04
CACTTTTCTTTNATCTCTGGTAAAGTTTCTTCTCCGTTTCTTTACAAAGTCTCCTACGAT
TCAAAACAGAGCAATGAGTATTTGGAACATAGTGTAGTATTAGTAGTCATACCAATTCGC
ATTTATTCCATCTCAAGAACTGATTGTTATACTCTGTTGGATTTGTCTTTCCTAAGCTCT
ATCTAACACAATTTTTTGGGCAGTTCTTCTTAATACTCTCGTGCAGTATCGCGGTTGGGA
CCAATCTCAGCCAATTCATCTGTATTGGAAGATTCACGGCGGTGTCTTTCCAAGTACTTG
GTCACATGAAGACAATTCTGGTTTTGGTTTTGGGATTCACTTTCTTCGGTAAGGAAGGAT
TAAACCTACAAGTAGTGCTTGGAATGCTGATTGCTATACTCGGTATGATCTGGTACGGTA
ATGCATCTTCTAAACCGGGTGGTAAAGAACGTCGTAGCCTGTCTATTCCCATAACCAAGT
CACAGAAACTACATGTAAATATGGATCCACATGTAAATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATTCACG

Last Updated on 06.12.2018 16:11