Sequences from confirmation amplicon


Confirmation sequences for line 828E11 and hit At5g09590 are shown.
Line ID 828E11
Locus At5g09590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2977406
Primers used for PCR EP33 - 8474, show primer details
   
Sequence Name 37-K103105-0022-828-E11C-EP33
GenBank Accession LN502577 [GenBank]
Sequencing primer gene specific primer, EP33: 5'-AAGCTTACCTTGGAAAATCTGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103105-0022-828-E11C-EP33
TTTATGATGCTCAGAGACAAGCAACAAAAGATGCTGGTAGAATTGCTGGTCTTGATGTTG
AGAGAATCATAAATGAACCGACGGCTGCTGCTCTGTCCTATGGAATGACCAACAAGGAGG
GGTTGATTGCTGTGTTTGATCTTGGTGGTGGGACATTTGATGTATCTGTTTTGGAGATTT
CTAATGGGGTATTCGAGGTAATTATCTCCCATGTGATCTTATGTTTGTCAGGTCTTCTGT
TGCTTTTTCTTTGAGAATCGTTCTTATTATTAAATTGCTTTTTTCAGGTGAAAGCCACCA
ATGGTGATACCTTCTTGGGAGGTGAGGATTTCGACAATGCTCTGCTAGACTTCTTGGTTA
ATGAGTTCAAGACCACTGAGGGAATAGACCTAGCCAAAGACAGACTTGCTCTGCAGAGGC
TTAGAGAAGCCGCTGAGAAAGCGAAGATTGAACTGTCATCTACTTCTCAAACTGAAATTA
ATCTCCCATTTATCACAGCCGATGCATCTGGAGCAAAGCATTTCAACATCACCCTAACAA
GGTCAAGGTTTGAAACTCTTGTGAATCACTTGATTGAGAGGACCCGCGATCCTTGCAAGA
ACTGTCTCAAGGATGCTGGTATAAGTGCTAAGGAAGTTGATGAGGTTCTTCTAGTTGGAG
GAATGACGCGTGTCCCCAAGGTTCAGTCTATTGTTGCGGAGATCTTTGGAAAGAGTCCAA
GCCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGT
   
Sequence Name 37-K103106-0022-828-E11C-8409
GenBank Accession LN502578 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103106-0022-828-E11C-8409
TTGAATATGGCTTGGACTCTTTCCAAGATCTCCGCAACAATAGACTGAACCTTGGGGACA
CGCGTCATTCCTCCAACTAGAAGAACCTCATCAACTTCCTTAGCACTTATACCAGCATCC
TTGAGACAGTTCTTGCAAGGATCGCGGGTCCTCTCAATCAAGTGATTCACAAGAGTTTCA
AACCTTGACCTTGTTAGGGTGATGTTGAAATGCTTTGCTCCAGATGCATCGGCTGTGATA
AATGGGAGATTAATTTCAGTTTGAGAAGTAGATGACAGTTCAATCTTCGCTTTCTCAGCG
GCTTCTCTAAGCCTCTGCAGAGCAAGTCTGTCTTTGGCTAGGTCTATTCCCTCAGTGGTC
TTGAACTCATTAACCAAGAAGTCTAGCAGAGCATTGTCGAAATCCTCACCTCCCAAGAAG
GTATCACCATTGGTGGCTTTCACCTGAAAAAAGCAATTTAATAATAAGAACGATTCTCAA
AGAAAAAGCAACAGAAGACCTGACAAACATAAGATCACATGGGAGATAATTACCTCGAAT
ACCCCATTAGAAATCTCCAAAACAGATACATCAAATGTCCCACCACCAAGATCAAACACA
GCAATCAACCCCTCCTTGTTGGTCATTCCATAGGACAGAGCAGCAGCCGTCGGTTCATTT
ATGATTCTCTCAACATCAAGACCAGCAATTCTACCAGCATCTTTTGTTGCTTGTCTCTGA
GCATCATTAAAGTAAGCTGGAACAGTGACAACAGCTTTCGTGACAGATTTTCC

Last Updated on 06.12.2018 16:11