Sequences from confirmation amplicon


Confirmation sequences for line 829B05 and hit At3g47340 are shown.
Line ID 829B05
Locus At3g47340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17440912
Primers used for PCR GE45 - 8474, show primer details
   
Sequence Name 12-K100994-0022-829-B05C-8409
GenBank Accession LN491501 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K100994-0022-829-B05C-8409
TCGGAATGAGCCATTTACATTGAACAATAATTTTCTTATTTTGAAAACCAAAAAACAACA
GAGGAAAAATGTATTGCCTGCGAGAAAGCTCAAGAACACGAACTCTCTTGGCCTGAGAAT
CATCGGAACATCCTAACACGGCAAGTATTCCACACATGTTTTTTTTTTGAAGAAAGTGAA
AAAGATCACGAAAAAGGAGAACAGAAAAACAGAATTTTCCGAAACTTTAGGGGATAGATA
TTGTAAAACCCCAAAAAGAGATTACAAGAAAATGTAATAAACAGACGGATGTCCGGGAAG
AGAAAGGAGAAAATTGCACGTAAAAGTGTACTATCGATATTCGGGTTTTTGATGTCTGGG
TTTTCTACAACCGATGGCTCTATTTATAGACACTAAACGGCGCCTTGATGTCGCGTTTGG
TTCTAAAGGAGTCGTGTATGGGAACAAATTATTTTTTTACTTGTAACCGGATAAGTTACC
GGATAACTCTAACCAGCTGTTTCCACGTGTTAATTAACCTTCTTACAACGTCCGATGGGT
TCTTACTTCTAAGTCTTTACATTCTCTTTTGTCCGGTTTCTTCCAATTTTGACGGTGGGG
CAAACTTCCGGTTTAGGGTTTATCCGGCTGAGTCAGCAAGTGGATAGGTACTTATCTGAC
GGTGTAAAAACTGTCCAATGATTGTTCTGATGGCTTTAGAGCCG
   
Sequence Name 12-K100998-0022-829-B05C-GE45
GenBank Accession LN491502 [GenBank]
Sequencing primer gene specific primer, GE45: 5'-GTGATAACACGTGTACGGCTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K100998-0022-829-B05C-GE45
TTTTTCCCGTCAGATAGTACCTATCCACTTGCTGACTCAGCCGGATAAACCCTAAACCGG
AAGTTTGCCCCACCGTCAAAATTGGAAGAAACCGGACAAAAGAGAATGTAAAGACTAAGA
AGTAAGAACCCATCGGACGTCGTAAGAAGGTTAATTAACACGTGGAAACAGCTGGTCAGA
GTTATCCGGTAACTTATCCGGTTACAAGTAAAAAAATAATTTGTTCCCATACACGACTCC
TTCAGAACCAAACGCGACATCACGGCGCCGTTTAGTGTCTATAAATAGAGCAATCGGTCG
TAGAAAACCAAGACATCAAAAACACGAATATCGATAGTACACTTCTACGTGCAATTTTCT
CCTTTCTCTTCCTGGACATCTGTCTGTTTATTACATTTTCTTGTAATCTCTTTTTGGGGT
TTTACAATATCTATCCCCTAAAGTTTCGGAAAATTCTGTTTTTCTGTTCTCATTCTTCGT
GATCTTTTTCACTTTCTTCAAAAAAAAAACATGTGTGGAATACTTGCCGTGTTAGGATGT
TCCGATGATTCTCAGGCCAAGAGAGTTCGTGTTCTTGAGCTTTCTCGCAGGCAATACATT
TTTCCTCTGTTTTTTTTTGGTTTTCAAAATAAGAAAATTATTTGTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11