Sequences from confirmation amplicon


Confirmation sequences for line 829C09 and hit At5g02940 are shown.
Line ID 829C09
Locus At5g02940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:685687
Primers used for PCR 37lx - 8474, show primer details
   
Sequence Name 70-K114191-0022-829-C09C-37lx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 37lx: 5'-AAGACTCATAACTTCCTCTGCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114191-0022-829-C09C-37lx
TGAGGTTAATAGCCCCGAACCCTACCTTACCTCCAGGCTGGTTGCAAATGGTTCCGCCCG
GGAAAAAAGTAAACCCAAATGGTTTGCCTCCCCAAAAGGGATCATAAAAACGCCAAATTT
TCCCTAAGTTGAACCCTTCATAATGGCCAAAAACGAAACCAAGAAAAAAAAACCAGGAGG
CTTCCCCCCAAAAAGCAAAGGGCTACCCAATTGCCAATGGTCCATTATTCCCATGGTCCT
TAATGGAAAAGGCCTCCTTGCCCGGAAAACCCCCTGGAATCCCCAAGGGTTATAATGGCC
AAAAGGGAGAAAAAAAAAAATTATTTCCCAATTTTTTTCCATTCCAAAAAGGAAAATTTC
CCCAGCGTAAAAAAAA
   
Sequence Name 35-K114190-0022-829-C09C-8409
GenBank Accession LN501918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114190-0022-829-C09C-8409
ATTTCATAATGAACATCGCATATTGAACAATCGCATATAGGTAAGCCATTTGCTTCTTGG
AGGTAAGCATCATGATTTATTCTTCTTGATTCTGTCTTTGGCAATAATGAATGATCAAAC
TAACGAATAGTTTGACGTCTTATAGATCCATCTAGAGCAGACAAACAATCTGCTTATACT
CTTCTCCTTGCTGAAACCATCTGCAACAAGCTTGGAGTTAAGGTAGTGTTAGTGACTATT
AACCTCATAATGCTACTGCCTATACTATTCACATCCTACTTCACTGAACTTTCATCTTAC
TATTGTTGTTTCTTTAGGTACATAATCTAGCCTCTGAAATTGTCGACACAAAACTCGGCA
AGCAGGTATTCAAAGGCTTTTTTTTTTTCCGGTCCCGGTAACTTTTTTACCGGTAAATTT
GCTAAACCTGGAAACAAAACCTTCCCAAAAAAAAAGATTCAGGCCGTCTTTGATTTTCAA
AGCAGCAAAGGATTTTTTGTTTT

Last Updated on 06.12.2018 16:11