Sequences from confirmation amplicon


Confirmation sequences for line 829G12 and hit At4g02920 are shown.
Line ID 829G12
Locus At4g02920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1293970
Primers used for PCR 26wu - 8474, show primer details
   
Sequence Name 49-K118761-0022-829-G12C-26wu
GenBank Accession LN506996 [GenBank]
Sequencing primer gene specific primer, 26wu: 5'-GAAAAGGTGATGGAGTTTCTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118761-0022-829-G12C-26wu
GTCGCTGTTAAATGAATTGATGGAGTTTGAGTCTATGAAATCCAGTTCTCAGCTACGACC
GTATGATACTTCCTCTGTTCTTTACTTGAATCAAGAATTAGGGAAACCGGTTTTGGATCT
CGTTAGGGATATGATGGAGAATCCAGAGTTCTCTGTGCGATCGAATGGTCATGTTCTGTT
CTCTTCAAGTAGCAATCCTGAGTTGAATGATCTACTTTCTATTGCTTCCGAGTTCAATTT
GTCAAGGAATTCAACAACAAAATGGAGACAGCTCTCACCGCTTATCCCACACTTTCAGAG
GTAAAATCCATCGCCCAGATTTATGCTCGCTTGTGTTGCGTTGATTTCTCGGTAAATGGA
TCAGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATAT
CCGCACGATTATT
   
Sequence Name 24-K118760-0022-829-G12C-8409
GenBank Accession LN497202 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118760-0022-829-G12C-8409
CTGATCCATTTACCGAGAAATCAACGCAACACAAGCGAGCATAAATCTGGGCGATGGATT
TTACCTCTGAAAGTGTGGGATAAGCGGTGAGAGCTGTCTCCATTTTGTTGTTGAATTCCT
TGACAAATTGAACTCGGAAGCAATAGAAAGTAGATCATTCAACTCAGGATTGCTACTTGA
AGAGAACAGAACATGACCATTCGATCGCACAGAGAACTCTGGATTCTCCATCATATCCCT
AACGAGATCCAAAACCGGTTTCCCTAATTCTTGATTCAAGTAAAGAACAGAGGAAGTATC
ATACGGTCGTAGCTGAGAACTGGATTTCATAGACTCAAACTCCATCAATTCATTTAACAG
CGACATATCGAATCCTTTTTCCTTGAACTCCTCTGACTGAGACAGAAGAAACTCCTCCAC
CTTTT

Last Updated on 06.12.2018 16:11