Sequences from confirmation amplicon


Confirmation sequences for line 832B07 and hit At5g55950 are shown.
Line ID 832B07
Locus At5g55950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22658966
Primers used for PCR 35jx - 8474, show primer details
   
Sequence Name 84-K113379-0022-832-B07C-35jx
GenBank Accession LN514454 [GenBank]
Sequencing primer gene specific primer, 35jx: 5'-CCAGTCTCAAACACAATAGGTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K113379-0022-832-B07C-35jx
GATTCTAGCTAAGCTTGAAGCTTTTGTTCTTCTTTGTTTCCAGCGTTGGATTCTATCAAA
TGGCTAAGATCGCAGTTACACCAACGATTGTTCTTGCCGAGTTTGTTCTCTTCAAGAAAA
CCATTTCTTCCACAAAGGTTTCGCTTATATCTTTTATGACATGTCAACAAAACAATCAAG
AATTCATTACATGTTTTTGGTGCTTACTCTGTTTTTGCAATTTGTCCTCTTACAGGTCAT
GGCATTAGCTGTAGTATCTCTAGGTGTGGCAATCGCGACAGTGACGGATCTAGAATTTAA
TTTGTTTGGTGCATTAGTTGCTGTTGCTTGGATTATTCCAAGTGCCATTAACAAAATACT
CTGGTCTAATCTTCAGCAACAAGCTAATTGGACTGCCCTAGCGTAAGAAATAAGAATCTC
TCTCTTTTACTTTTACTTGAGGTGTAAGCAATACAAGTCTCTTAAAATAAAGGCTTTATT
GAATGTAGATTGATGTGGAAGACAACACCGTTTACCAATTGATGCAAACATGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 46-K113377-0022-832-B07C-8409
GenBank Accession LN505922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K113377-0022-832-B07C-8409
ATGTTTGCATCAATTGGTAACGGTGTTGTCTTCCCATCAATCTACATTCAATAAAGCCTT
TATTTTAAGAGACTTGTATTGCTTACACCTCAAGTAAAAGTAAAAGAGAGAGATTCTTAT
TTCTTACGCTAGGGCAGTCCAATTAGCTTGTTGCTGAAGATTAGACCAGAGTATTTTGTT
AATGGCACTTGGAATAATCCAAGCAACAGCAACTAATGCACCAAACAAATTAAATTCTAG
ATCCGTCACTGTCGCGATTGCCACACCTAGAGATACTACAGCTAATGCCATGACCTGTAA
GAGGACAAATTGCAAAAACAGAGTAAGCACCAAAAACATGTAATGAATTCTTGATTGTTT
TGTTGACATGTCATAAAAGATATAAGCGAAACCTTTGTGGAAGAAATGGTTTTCTTGAAG
AGAACAAACTCGGCAAGAACAATCGTTGGTGTAACTGCGATCTTAGCCATTTGATAGAAT
CCAACGCTGGAAACAAAGAAGAACAAAAGCTTTCAAGCTTAAGCTATGAATCTTGATTCA
AACTTTAGCGATTGAGCTATGAACCTATTGTGT

Last Updated on 06.12.2018 16:11