Sequences from confirmation amplicon


Confirmation sequences for line 835B03 and hit At5g43100 are shown.
Line ID 835B03
Locus At5g43100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17302514
Primers used for PCR 05ja - 8474, show primer details
   
Sequence Name 31-K112831-0022-835-B03C-05ja
GenBank Accession LN500236 [GenBank]
Sequencing primer gene specific primer, 05ja: 5'-GCATATTTTGTAACATCCCACGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112831-0022-835-B03C-05ja
ATTTCATTAACAATATTACTTAACAACTAAAAATCATAAAATATCACTTAAATTCACTAA
ATTTAATTTCTCAATAAAATCATAAAATATCACTTAAACACATATCTCTCACAAACCAAA
TTAAACCCTCACGCCAATAAGCCTTCCAAATACATTCCAACCATTTTTCTCTCTATTTTC
TTTCTTTCCTTGTACATCAAAATAGCGTAATAACTACATCAATTTACTTGAAATCAAAAG
TGTTTTTAATTTGAGGAATCTTGTTTCCTGTGTTCTGGGGTTTCATTTTCTTTCTATCAA
ATATATAAATATATATAAAGAGCGAGAGACCGTAAAGAGACTCTATGAGTGATGAGGAGG
CTCAGGCATTAGATGTTTCAGAGGAGAGAGGCTGAAGTTCTTGTTCTTTAATAGCAGCAT
TTACAGGCTCATAAGTAGCTTCTTCTTGCTTTCGCCGTCTCCACACCAATGCAAGTTTGA
TCATCACCGAAGTAACAAGCAACGATATCATCGCTCCTCCCACCACACCAAGTAAGTGTT
TCTCCCACCAGCTCCTGCTTAAAGCAAACATTACTGTTTCTTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
   
Sequence Name 02-K112829-0022-835-B03C-8409
GenBank Accession LN487108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112829-0022-835-B03C-8409
CATTTCATTGAAGAACGTAATGTTTGCTTTAAGCAGGAGCTGGTGGGAGAAACACTTACT
TGGTGTGGTGGGAGGAGCGATGATATCGTTGCTTGTTACTTCGGTGATGATCAAACTTGC
ATTGGTGTGGAGACGGCGAAAGCAAGAAGAAGCTACTTATGAGCCTGTAAATGCTGCTAT
TAAAGAACAAGAACTTCAGCCTCTCTCCTCTGAAACATCTAATGCCTGAGCCTCCTCATC
ACTCATAGAGTCTCTTTACGGTCTCTCGCTCTTTATATATATTTATATATTTGATAGAAA
GAAAATGAAACCCCAGAACACAGGAAACAAGATTCCTCAAATTAAAAACACTTTTGATTT
CAAGTAAATTGATGTAGTTATTACGCTATTTTGATGTACAAGGAAAGAAAGAAAATAGAG
AGAAAAATGGTTGGAATGTATTTGGAAGGCTTATTGGCGTGAGGGTTTAATTTGGTTTGT
GAGAGATATGTGTTTAAGTGATATTTTATGATTTTATTGAGAAATTAAATTTAGTGAATT
TAAGTGATATTTTATGATTTTTAGTTGTTAAGTAATATTTGTTAATGAAATTCACTAACT
TATATCTCTATTAGCGAAATAACCCGATGTTACGTGGGATGTTACAAAATATG

Last Updated on 06.12.2018 16:11