Sequences from confirmation amplicon


Confirmation sequences for line 835C03 and hit At3g28956 are shown.
Line ID 835C03
Locus At3g28956 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10981928
Primers used for PCR 30qp - 8474, show primer details
   
Sequence Name 78-K115691-0022-835-C03C-30qp
GenBank Accession LN513730 [GenBank]
Sequencing primer gene specific primer, 30qp: 5'-ATACGCGTTTAAGTGGATGGTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K115691-0022-835-C03C-30qp
CAAGTCTAGAGCTGTCAAATGGTCCGTCCAGGGACCATTGGGCGTGTACGTACAGACAAT
TTTTTTTTATGGGCCAAGTTGGACGGTCCATGGACAATGGTCCAAAAAATGTCCATGCCC
AATAAAGACCATGTTTATATAGAAAGTCTATGGTCAGCCCATTTTATTATTGTTATTATT
GATAAATCATAAATCATAATTTTTAAGAAAAATTGAGAAATTACATTTTTCTGCAAAAAT
TGTAAAAATCGGAGACTTGCCTTTTTCCGCCAAAACGGTAAAATCGCACCGATACAAAAA
ATAACGATTTAAGGGGAAAATTGTTGCAAGTCACGACTGTTCACCATTCTCTGTTTCTTC
TTCTTCATCATTATCTTTAGGACTTTCAGCTGTAGGCAGAGGAGGTAGCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 30-K115690-0022-835-C03C-8409
GenBank Accession LN499857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115690-0022-835-C03C-8409
ATTGCTACCTCCTCTGCCTACAGCTGAAAGTCCTAAAGATAATGATGAAGAAGAAGAAAC
AGAGAATGGTGAACAGTCGTGACTTGCAACAATTTTCCCCTTAAATCGTTATTTTTTGTA
TCGGTGCGATTTTACCGTTTTGGCGGAAAAAGGCAAGTCTCCGATTTTTACAATTTTTGC
AGAAAAATGTAATTTCTCAATTTTTCTTAAAAATTATGATTTATGATTTATCAATAATAA
CAATAATAAAATGGGCTGACCATAGACTTTCTATATAAACATGGTCTTTATTGGGCATGG
ACATTTTTTGGACCATTGTCCATGGACCGTCCAACTTGGCCCATAAAAAAAAATTGTCTG
TACGTACACGCCCAATGGTCCCTGGACGGACCATTTGACAGCTCTAGACTTGCTAATGAA
GTTTTTGAGTTTGTTATCTTTTAGATATAAAGAACATATTTTGATTTTGTAACCATCC
Primers used for PCR 68xb - 8474, show primer details
   
Sequence Name 69-K118893-0022-835-C03C-68xb
GenBank Accession LN512293 [GenBank]
Sequencing primer gene specific primer, 68xb: 5'-CTTGCTAGCTTTATCGGTTAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118893-0022-835-C03C-68xb
TATTTGATAAAGATACAATTGAGGGTTTAATACAATTGTAAGAGGCCTAAACCATAGCCA
TTAAACCGAACTAAACCAACACTATCTACACTGGTTTATACAAAATACCAGTTCACAATG
ACTCACTAAGACTCTAATTACAACAGAGACTCTATATACACTAATAGATTGAGGTCTCAC
ATCTTCTTTGCTTGTTCTTAGGGTCTTCATGAATTGAAGCACATCAATATCTTGGTCGAA
AGATTCCCCCTTTCAGGTATGTAGAGACCCTTTGTGTTGAAATCTGAATTTGATTCCATA
GAGCTAAGATTCATCACTTTTGATATGGATTTTGGACTCCAAAGAGATTCTGAAATGAAT
AGGCTCAGTTTTTGTATTGGATTTTCTCAGGTCTCTATCACAAAGGTGCTCATCGATTCC
TCTGTTTTTTGTTTTCTTTTGAAATTTTCTTTTATGATCATTGGATTTTGATACCATTCA
TTACTGATCATTGATCGATGAGTATGATCCTTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 29-K118892-0022-835-C03C-8409
GenBank Accession LN499500 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118892-0022-835-C03C-8409
TTGAAGGATCATACTCATCGATCAATGATCAGTAATGAATGGTATCAAAATCCAATGATC
ATAAAAGAAAATTTCAAAAGAAAACAAAAAACAGAGGAATCGATGAGCACCTTTGTGATA
GAGACCTGAGAAAATCCAATACAAAAACTGAGCCTATTCATTTCAGAATCTCTTTGGAGT
CCAAAATCCATATCAAAAGTGATGAATCTTAGCTCTATGGAATCAAATTCAGATTTCAAC
ACAAAGGGTCTCTACATACCTGAAAGGGGGAATCTTTCGACCAAGATATTGATGTGCTTC
AATTCATGAAGACCCTAAGAACAAGCAAAGAAGATGTGAGACCTCAATCTATTAGTGTAT
ATAGAGTCTCTGTTGTAATTAGAGTCTTAGTGAGTCATTGTGAACTGGTATTTTGTATAA
ACCAGTGTAGATAGTGTTGGTTTAGTTCGGTTTAATGGCTATGGTTTAGGCCTCTTACAA
TTGTATATAAACCCTCAATTGTATCTTTTATCAAATTAATGAGAATGACATTAACTTGGT
AATATGATCCATTAACCGATAAAGCTAGCAA

Last Updated on 06.12.2018 16:11