Sequences from confirmation amplicon


Confirmation sequences for line 836B11 and hit At1g16890 are shown.
Line ID 836B11
Locus At1g16890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5777595
Primers used for PCR 13wl - 8474, show primer details
   
Sequence Name 60-K118635-0022-836-B11C-13wl
GenBank Accession LN510377 [GenBank]
Sequencing primer gene specific primer, 13wl: 5'-AAGGAACCGACGGATTATATACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118635-0022-836-B11C-13wl
GATCTTATATCATCTTCGTCATCTTGATCTGACTTTTTTTTGTTCTTCGATCTGATAGGA
AACTCAACGTCTGCTTAGTGAACCTGGTAATTTCATCACTTCATTCGATCCTTGTTGATT
TCTCACAGTTGATTTTAGCTCAAACAGTTGGTTTTGGTAGCTGGAACTATTGATTTTAAT
TCTAAGTTGTATATCTTTCGAAATTGCAGCTCCTGGGATAAGTGCGTCTCCATCAGAGGA
AAATATGAGATATTTCAATGTTATGATTCTTGGTCCTACTCAATCTCCTTATGAAGGTAG
TCAAATTGTTTTAATCTCCCAAAGATGCTCATCGTTACTTCTTCCTTTTTCTTCTTTAAT
GCATGCTGCTCAACGATTTATCGTTATCGTTTCTGCTGCAAGTAGTGACTCTGCTCTGTC
TCTATATTTGTTTATACAAACGGATATGTTCGTTTAGGTACTACTAGGTCTCATAGATTG
CTCATGTTCGGATTGTAGTGTTATGAATAACGATGAGTCTTTAAGCTACCTTTGAGTTCA
TTTCGACACATTTTATTTGTTCTACACAATATGGAGATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGGTTATTA
   
Sequence Name 20-K118634-0022-836-B11C-8409
GenBank Accession LN495426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118634-0022-836-B11C-8409
ACAATAAATGTGTCGAATGAACTCAAGGTAGCTTAAAGACTCATCGTTATTCATAACACT
ACAATCCGAACATGAGCAATCTATGAGACCTAGTAGTACCTAAACGAACATATCCGTTTG
TATAAACAAATATAGAGACAGAGCAGAGTCACTACTTGCAGCAGAAACGATAACGATAAA
TCGTTGAGCAGCATGCATTAAAGAAGAAAAAGGAAGAAGTAACGATGAGCATCTTTGGGA
GATTAAAACAATTTGACTACCTTCATAAGGAGATTGAGTAGGACCAAGAATCATAACATT
GAAATATCTCATATTTTCCTCTGATGGAGACGCACTTATCCCAGGAGCTGCAATTTCGAA
AGATATACAACTTAGAATTAAAATCAATAGTTCCAGCTACCAAAACCAACTGTTTGAGCT
AAAATCAACTGTGAGAAATCAACAAGGATCGAATGAAGTGATGAAATTACCAGGTTCACT
AAGCAGACGTTGAGTTTCCTATCAGATCGAAGAACAAAAAAAAGTCAGATCAAGATGACG
AAGATGATATAAGATCGTACAAGCTATCGAAAATTCTTCCCAGTTCTGCTGAGGTATAT

Last Updated on 06.12.2018 16:11