Sequences from confirmation amplicon


Confirmation sequences for line 838F01 and hit At2g16910 are shown.
Line ID 838F01
Locus At2g16910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7333916
Primers used for PCR 06ha - 3144, show primer details
   
Sequence Name 41-K122663-0022-838-F01C-06ha
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 06ha: 5'-CTTATTGGTTGTGGTAATGGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K122663-0022-838-F01C-06ha
TTCTCTGGTGATCAAATGGGTGATGATGACCATTGTGATGCTGGTGATCATCATAGTGTT
GATAATCAACTTCGATATCCATTGCAAAATGTACTTTCATTTTATAATAACGCTGCGGAC
ATCTACATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTGAC
CATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTTACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 38-K122735-0022-838-F01C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K122735-0022-838-F01C-3144
CTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGCAATGGATATCGAAGTTGATTATCAACACTATGATGATCAC
CAGCATCACAATGGTCATCATCACCCATTTGATCACCAGATGAATCAGAGCGCTCATCAT
CATCACCACCACCAACACATCAACCATTACCACAACCAATAAG
Primers used for PCR 40yx - 3144, show primer details
   
Sequence Name 12-K123023-0022-838-F01C-40yx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40yx: 5'-TGATTCCTATACCGGGAGGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K123023-0022-838-F01C-40yx
GTCTCATAATGTTAAGTTTATGGTTTTTTGTATTGTGGTTGTTTGTGATGATGTGTGCGC
GTATAGGTCGCTGAAGATCAGAACGTGGTGGATTTTGTAATGGGACATTGCAACATGTTG
ATGGACGATTCTGTAACGATAAACATGATGGTAGCAGACGAGGTCGAATCGAAGCCGTAC
GGGATGTTATCCGGTGACATCCAGCAAAAGGGTTCCAAAGAAGAAGATATGATGAATCTC
CCTTCGTCTTACGATATCTCTGCTGATCAGATCCGACTCAATTTCTTGCCTCAGATGAGT
GATTACGAGACACAACACTTGAAGATGAAGAGTGATTATCATCATCAAGCCTTGGGGTAT
CTCCCAGAGAATGGTAACAAGGAAATGATGGGTATGAACCCATTTAACACGGTGGAAGAA
GATGGGATTCCAGTGATTGGAGAGCCTAGCTTGCTTGTGAATGAACAGCAAGTTGTTAAC
GATAAGGATATGAATGAGAACGGTAGGGTGGATTCAGGGTCGGATTGCAGCGACCAGATT
GATGATGAAGATGATCCAAAGTACAAGAAGAAGTCAGGAAAAGGATCTCAAGCCAAGAAC
CTGATGGCTGAGAGACGGAGGAGGAAGAAGCTAAACGATAGGCTTTATGCTCTCCGGTCG
CTTGTTCCCAGGATAACCAAGGTATCTTGGTTTTCGTCTCTGGGTTTACTTCTTGGTTTC
TGAGTTATAATGCGTTCTCTGAAACTAGTTTTTGTGGCATGGAAGTTGGACAGGGCGTCG
ATTCTTGGCGATGCAATCAACTACG
   
Sequence Name 02-K123022-0022-838-F01C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K123022-0022-838-F01C-3144
ATCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
ATGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCCATCTGATCATCTTGCCATCCTC
TAGATGTGTTCCGGGTTATTTCCAGCAACGAGTTCCTTACGTGTTCAGCTTGGACCATCT
CGTTATCATTTTTCTGTAACATGGGAAAACAGAAACATATAGTTTTTGTATATTTTGCTT
TGGTTTTTGTTTCTCCATTCCGCAGGTCCTTTAGATCATACCTCGACTTTGAAGACATTG
GAGACCAGGCTGAGGTAGCGAGTCGTGTTAGCATTTGTGACTTCTAGTCCAAGAGAATCC
AGTGCCTCCATTAGCCTTGTGAAGCCTCCTGGTTTGTATTCGCAAATCACCTTTACGAAA
AACTCTCTGCCATCTAACTGAGCCACGTCCACCTGTGGCTGAAACTCCGCAACCCAACAT
AAACCGATATCATGAGGTTTTGATTTTAGAGTTCAGATTAGAAGAAAGGTGAGAGTTAGT
TTCTTGTTATGATGCTCTAACCTCCATTTCTTGTCCTTTATCATTGGAATTCTCAAGATC
AACATCTTGCTTCACGCTAGGAACGTTGGAGTTACATGAAAGCCCCGGGTGAAACCCAGT
GACCACTGTCCCGTTCAGGCTCATCCCTCCTTGTGGCCTATTAGATCCATCCTCAGTCTC
TGAGTTCTCTTCAAGCTCGTCTTGAAGCTCCTTTGCCTCATTCTGCAACTCCTTAACGTA
GTTGATTGCATCGCCAAGAATCGACGCCCTGTCCCACTTCCATGCCACAAAAACTAGTTT
CAGAGAACGCATTATAACTC

Last Updated on 06.12.2018 16:11