Genotyping and primer details


Genotyping and primer details for 838F01 - At2g16910
Line ID 838F01
Locus At2g16910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7333916 of line 838F01 Chr2:7333916
   
Insertion allele amplicon 3144 - 06ha
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 06ha: 5'-CTTATTGGTTGTGGTAATGGTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7334078
   
Insertion allele amplicon 3144 - 40yx
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 40yx: 5'-TGATTCCTATACCGGGAGGACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7332374
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11