Sequences from confirmation amplicon


Confirmation sequences for line 839B07 and hit At2g32580 are shown.
Line ID 839B07
Locus At2g32580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13828258
Primers used for PCR 40ym - 8474, show primer details
   
Sequence Name 28-K120874-0022-839-B07C-40ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40ym: 5'-TACATACTTGGTCCACCGCTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K120874-0022-839-B07C-40ym
TTCTTGCTCTGCTTGCGTCTGTGATTGCTCTTCGTTGCCTCTTCTCACCATCCCAACTGG
TAATCACTATTTCCCTTTATTGGATCTTGCTTGATCGGCTCTAAAGTTTATTCATTTTCC
CTGAGACAACGAGAGCAAATTTAGTACCTTTTTCATATTTGGTATTGTTTAGTAGTAGTT
GGTGATTGAGTTGCCAGATTGCATGCTTTAGCTTGATGAGGGATTTTGAGTTTGAATCTC
TATGCAGATAGATAGACAAGAATTGTGTAGATCTACAAGAATTGTTTTATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTATTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K120873-0022-839-B07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K120873-0022-839-B07C-8409
CATTTCATTGAATATAAAACAATTCTTGTAGATCTACACAATTCTTGTCTATCTATCTGC
ATAGAGATTCAAACTCAAAATCCCTCATCAAGCTAAAGCATGCAATCTGGCAACTCAATC
ACCAACTACTACTAAACAATACCAAATATGAAAAAGGTACTAAATTTGCTCTCGTTGTCT
CAGGGAAAATGAATAAACTTTAGAGCCGATCAAGCAAGATCCAATAAAGGGAAATAGTGA
TTACCAGTTGGGATGGTGAGAAGAGGCAACGAAGAGCAATCACAGACGCAAGCAGAGCAA
GAAGTGGCTGAAACGGCTAAGGCTTCCGTGAGGTGCCAGTAGAGCGGTGGACCANAGTAT
GT

Last Updated on 06.12.2018 16:11