Sequences from confirmation amplicon


Confirmation sequences for line 839D11 and hit At3g26480 are shown.
Line ID 839D11
Locus At3g26480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9689050
Primers used for PCR DM06 - 8474, show primer details
   
Sequence Name 87-K101748-0022-839-D11C-8409
GenBank Accession LN514745 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101748-0022-839-D11C-8409
GAGCCATTTACATTGATTTTACTATTTAACAAACCTAACATATGCAGGTTCAAATTTGCG
ATCATCATCCAGACGATGATGATCAAGTCCGGGTTAGTACATTTTGTTTCTTCTCTGTAA
GATGACATAGTTGTTACTAGTAAAAGATCCGCTCTCTTTCTGTTTTGTAATAATTTGAAG
AATTATGGAAAATAGGTTCTCGTGACTGCGGTTGCTCTCTCTCGGAATGGCTCAGTAATG
ACTACAGCTGAGGCCAGATTTGCTGCAAGCAATTTCAGTGAAGGCATAGTTTCTCTCAAG
TTTTGGGTATTTGTACCGGACAGCAAAACTTTCAGCTTATCGACAGTCATAAATCAACCT
CACAGGTTTTTACTTCAATGTTCTTATAAACTTTTAAACTCCTTGATTCTCATCGTGAGT
GTAACTTTTGATCCTTCTGTATTCTCACAGGGAAGCTGCAATTACAGCCATTGCCCTTAA
CCCCGCCCGTTCCATGGCTGTTAGTATATCTTCTGCTGGGGACTTCAAGGTAGGATTACA
GAGTAGTGAAAAATGGATCATTTCCTTGGAATATCGTTTGACCTTTTGTG
   
Sequence Name 87-K101754-0022-839-D11C-DM06
GenBank Accession LN514746 [GenBank]
Sequencing primer gene specific primer, DM06: 5'-ACACAAAAGGTCAAACGATATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101754-0022-839-D11C-DM06
TCTGTATCCTACCTTGAAGTCCCCAGCAGAAGATATACTAACAGCCATGGAACGGGCGGG
GTTAAGGGCAATGGCTGTAATTGCAGCTTCCCTGTGAGAATACAGAAGGATCAAAAGTTA
CACTCACGATGAGAATCAAGGAGTTTAAAAGTTTATAAGAACATTGAAGTAAAAACCTGT
GAGGTTGATTTATGACTGTCGATAAGCTGAAAGTTTTGCTGTCCGGTACAAATACCCAAA
ACTTGAGAGAAACTATGCCTTCACTGAAATTGCTTGCAGCAAATCTGGCCTCAGCTGTAG
TCATTACTGAGCCATTCCGAGAGAGAGCAACCGCAGTCACGAGAACCTATTTTCCATAAT
TCTTCAAATTATTACAAAACAGAAAGAGAGCGGATCTTTTACTAGTAACAACTATGTCAT
CTTACAGAGAAGAAACAAAATGTACTAACCCGGACTTGATCATCATCGTCTGGATGATGA
TCGCAAATTTGAACCTGCATATGTTAGGTTTGTTAAATAGTAAAATCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11