Sequences from confirmation amplicon


Confirmation sequences for line 845C06 and hit At3g60790 are shown.
Line ID 845C06
Locus At3g60790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22466918
Primers used for PCR 39uj - 8474, show primer details
   
Sequence Name 31-K117387-0022-845-C06C-39uj
GenBank Accession LN500322 [GenBank]
Sequencing primer gene specific primer, 39uj: 5'-TTTTGTTTTGACATGGTTTGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117387-0022-845-C06C-39uj
GATTTGGCTTGGCTTATGGTGAGCCAAATTTATCATATGAAACGGACTGGATCCTTATCC
GTGAGTGTCGATGTGAGAAATCCGAACGAAGTCGAGATTTTGAAGGAAGTCTTACTTTTG
TGGGATGGAGAGATGATGGATCTGGAGATCCTCTTCAAGGTACATTTTACTTGATGGTTT
TTTTTTTCTATGTGGATAAATAATTTGATCACTCACATGTCATATTTGCATTTTAAAACA
ACAATGCTCCTATAGAAAAAGGTGAATCTTTTATTACTGGTGGAGCACGTAACAAATGGT
GGGATGGTGAAAAACCGTTTCCAAATGATTTCTTCCGCGTTTGTACTGTGTGGATGTATA
ATTTCAATGGTTCTAATGAAAAGGAATTTGCATTAGCGTCACGTTTTGTGACACAAGGAA
CAGTGACTGAAAAGTTGATGATAAAGACATCGACTTATCCTCCAGTGAAGCATTTAATGA
CAGAAGCAAAAGTGGCCAAGCTAATGGAACTTCCTAAAGGTGATGCTTTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTC
   
Sequence Name 04-K117386-0022-845-C06C-8409
GenBank Accession LN488088 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117386-0022-845-C06C-8409
ATTTCATTGAAGCATCACCTTTAGGAAGTTCCATTAGCTTGGCCACTTTTGCTTCTGTCA
TTAATTGCTTCACTGGAGGATACGTCGATGTCTTTATCATCAACTTTTCAGTCACTGTTC
CTTGTGTCACAAAACGTGACGCTAATGCAAATTCCTCTTCATTAGAACCATCGAAATTAT
ACATCCACACAGTACAAACGCGGAAGAAATCATCTGGAAACGGTTTTTCACCATCCCACC
ATTTGTTACGTGCTCCACCAGTAATAAAAGATTCACCTTCTTCTATAGGAGCATTGTTGT
TCTAAAATGCAAATATGACATGTGAGTGATCAAATTATTTATCCACATAGAAAAAAAAAA
CCATCAAGTAAAATGTACCTTGAAAAGGATCTCCAAATCCATCATCTCTCCATCCCACAA
AAGTAAAACTTCCTTCAAAATCTCAACTTCGTTCGGATTTCTCACATCGACACTCACGGA
TAAGGATCCAGTCCGTTTCATATGATAAATTTGGCTCACCATAAGCTCAAGCCAAATTCT
TATTTTCTCCTGAATTCATAATACAACAAACCATGTAAAAACCAA

Last Updated on 06.12.2018 16:11