Sequences from confirmation amplicon


Confirmation sequences for line 846C06 and hit At5g06980 are shown.
Line ID 846C06
Locus At5g06980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2167997
Primers used for PCR CS23 - 8474, show primer details
   
Sequence Name 78-K031321-022-846-C06C-8409
GenBank Accession LN513625 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K031321-022-846-C06C-8409
CATTTACATTGAAGCCTATCCATCTCCATCTGACTGTATAATCCTCCACTGAAACCATTG
TGAATCACATCATAATCAAAACATTTCTCAGCAGCTTCTGAAGAGTTCATGCTCCATCCA
GTACCCCACATATCAGGAGAAGGGTACGAATCTGATGTCTCTTGATAGTTAGGCACAACA
AGATCCTCTAAATTCCTCCTCGAATAACGATCCATGAATGATACTTTAACCTGCTAACAA
GTCACTCACTATATTCAGCAACCAACAGGAGAAAAACATTTGAATTCTTAAGAATAAAAT
AATCAAAAGAAGTCACCTTTATGATCAGATCTAAATCCAAATCCAAAAGATTATGAGTCT
ATCTTCTTCTATCTTCTGTAGATTTGTGAGTGCGAAGGAGGAAAGAGAGAGAAGACAAAG
AAGCAAGTTGAAGAGAGGAAGTGGAGAGCGCGTGAGTTTGAACTTTAGTTAGTGGCTTAG
AATTCACAAACGAGATTTGTGGCTTAGATTACTCCTCCATCTAAAGCAACTCTT
   
Sequence Name 79-K031321-022-846-C06C-CS23
GenBank Accession LN513763 [GenBank]
Sequencing primer gene specific primer, CS23: 5'-GGACCAATGAAAGAGTTGCTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K031321-022-846-C06C-CS23
TTTGTGATTCTAGCCACTAACTAAAGTTCAACTCACGCGCTCTCCACTTCCTCTCTTCAA
CTTGCTTCTTTGTCTTCTCTCTCTTTCCTCCTTCGCACTCACAAATCTACAGAAGATAGA
AGAAGATAGACTCATAATCTTTTGGATTTGGATTTAGATCTGATCATAAAGGTGACTTCT
TTTGATTATTTTATTCTTAAGAATTCAAATGTTTTTCTCCTGTTGGTTGCTGAATATAGT
GAGTGACTTGTTAGCAGGTTAAAGTATCATTCATGGATCGTTATTCGAGGAGGAATTTAG
AGGATCTTGTTGTGCCTAACTATCAAGAGACATCAGATTCGTACCCTTCTCCTGATATGT
GGGGTACTGGATGGAGCATGAACTCTTCAGAAGCTGCTGAGAAATGTTTTGATTATGATG
TGATTCACAATGGTTTCAGTGGAGGATTATACAGTCAGATGGAGATGGATATGGCTTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCACTCCCCA

Last Updated on 06.12.2018 16:11