Sequences from confirmation amplicon


Confirmation sequences for line 848H11 and hit At1g07520 are shown.
Line ID 848H11
Locus At1g07520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2311438
Primers used for PCR CP49 - 8474, show primer details
   
Sequence Name 69-K031142-022-848-H11C-CP49
GenBank Accession LN512122 [GenBank]
Sequencing primer gene specific primer, CP49: 5'-AGATTTCGTTGCTGAATCTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K031142-022-848-H11C-CP49
TTCGGGAATTTAGGGTTTTACAAAAAAATAAAAAAGAGTTTCTTTTTTTGGATGATGGAA
TCGAATTACTCAGGTGTGGTCAACGGCTACGATGTGAGTTTCTTACCAACTTCGATTCCG
GATCTTGGATTCGGTGTTCCTTCTTCTAGTGATTTTGATCTTCGGATGGATCAATACTAT
CACCAACCTTCGATTTGGGTTCCAGATCAAGATCATCACTTTTCTCCACCAGCTGATGAG
ATTGATTCTGAAAACACCCTTTTGAAATATGTAAACCAGCTCCTTATGGAAGAGAGTCTC
GCTGAGAAGCAGTCTATCTTCTATGATTCCTTGGCTCTTCGACAAACCGAGGAAATGTTG
CAGCAAGTCATCAGCGACTCGCAGACTCAGTCTTCTATACCTAATAATTCGATTACTACC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACTT
   
Sequence Name 34-K031142-022-848-H11C-8409
GenBank Accession LN501298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K031142-022-848-H11C-8409
TTGAATAATCCTGGTAGTAATCGAATTATTAGGTATAGAAGACTGAGTCTGCGAGTCGCT
GATGACTTGCTGCAACATTTCCTCGGTTTGTCGAAGAGCCAAGGAATCATAGAAGATAGA
CTGCTTCTCAGCGAGACTCTCTTCCATAAGGAGCTGGTTTACATATTTCAAAAGGGTGTT
TTCAGAATCAATCTCATCAGCTGGTGGAGAAAAGTGATGATCTTGATCTGGAACCCAAAT
CGAAGGTTGGTGATAGTATTGATCCATCCGAAGATCAAAATCACTAGAAGAAGGAACACC
GAATCCAAGATCCGGAATCGAAGTTGGTAAGAAACTCACATCGTAGCCGTTGACCACACC
TGAGTAATTCGATTCCATCATCCAAAAAAAGAAACTCTTTTTTATTTTTTTGTAAAACCC
TAAATTCCCTGAATTTGGCTTCTCGACATACAAAGAACAGAGTTTTACAAAAGATTCAGC
ACCGAAATC

Last Updated on 06.12.2018 16:11