Genotyping and primer details


Genotyping and primer details for 848H11 - At1g07520
Line ID 848H11
Locus At1g07520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2311438 of line 848H11 Chr1:2311438
   
Insertion allele amplicon 8474 - CP49
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP49: 5'-AGATTTCGTTGCTGAATCTTTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2311917
   
wt amplicon (over InsPos) 09sl - CP49
wt amplicon start Chr1:2310996
wt amplicon end Chr1:2311917
wt amplicon size 922 bp
Locus specific primer CP49: 5'-AGATTTCGTTGCTGAATCTTTTGT-3'
2nd locus specific primer 09sl: 5'-TAATTTCCCATCTTCCTCATTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11