Sequences from confirmation amplicon


Confirmation sequences for line 850G03 and hit At2g16430 are shown.
Line ID 850G03
Locus At2g16430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7120896
Primers used for PCR BC75 - 8474, show primer details
   
Sequence Name 21-K104153-0022-850-G03C-BC75
GenBank Accession LN495636 [GenBank]
Sequencing primer gene specific primer, BC75: 5'-GCTTACATAATCGTGCTAGCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104153-0022-850-G03C-BC75
CCTTTTTGTGCATATTCTAAACCNATGAGTAGAGGATCTGAATATTCTTATCTTTCTTGG
ACAGGAAAATACACACCACAGTACCAATGGCTCGAAGAGGAGTTCCCAAAGGTTAACAGA
ACGGAAACTCCGTGGTTGATTGTTCTGATGCATTCACCATGGTACAACAGCTATGATTAC
CATTACATGGAAGGTGAAACAATGAGGGTAATGTATGAGGCTTGGTTTGTCAAGTACAAA
GTAGATGTAGTTTTTGCAGGTCATGTCCACGCCTACGAAAGATCGGTAAGATCAATAACT
TTTGGGTCACGATGTCTCTTTAAACTATAGATACTAAGAAATCTAAGCTATTGTGAAACC
AGGAACGTGTATCAAACAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 21-K104154-0022-850-G03C-8409
GenBank Accession LN495637 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104154-0022-850-G03C-8409
GCCATTTACATTGAATATATACTCATTGTTTGATACACGTTCCTGGTTTCACAATAGCTT
AGATTTCTTAGTATCTATAGTTTAAAGAGACATCGTGACCCAAAAGTTATTGATCTTACC
GATCTTTCGTAGGCGTGGACATGACCTGCAAAAACTACATCTACTTTGTACTTGACAAAC
CAAGCCTCATACATTACCCTCATTGTTTCACCTTCCATGTAATGGTAATCATAGCTGTTG
TACCATGGTGAATGCATCAGAACAATCAACCACGGAGTTTCCGTTCTGTTAACCTTTGGG
AACTCCTCTTCGAGCCATTGGTACTGTGGTGTGTATTTTCCTGTCCAAGAAAGATAAGAA
TATTCAGATCCTCTTACTCATGGTTTGAGAATATTGCACAAAAAGGTTTAATATTTTTAA
AGATGCCTTGCCATATGCTGAATATGAAGCTAGCACGATANTGTAAG

Last Updated on 06.12.2018 16:11