Sequences from confirmation amplicon


Confirmation sequences for line 851E09 and hit At1g12150 are shown.
Line ID 851E09
Locus At1g12150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4125149
Primers used for PCR 08ty - 8474, show primer details
   
Sequence Name 34-K117118-0022-851-E09C-08ty
GenBank Accession LN501571 [GenBank]
Sequencing primer gene specific primer, 08ty: 5'-GAGAATGTTTGTGTATGCAGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117118-0022-851-E09C-08ty
AAAAGCTCCGGAATCTCCAAGGACAATGGAGGTGGGAAAAAATAAACACAAGAGCACCGT
TCCAATCGGTGAAAGCTGCGGTGAGCTTGTTCGGAAAATTTGCAGTCTCAAAGCAAAGAA
TTACTCCAAGGCGATCTCGACTCTCCTCTGAGGTAAGTAATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATTTC
   
Sequence Name 12-K117117-0022-851-E09C-8409
GenBank Accession LN491761 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117117-0022-851-E09C-8409
TTGAATTACTTACCTCAGAGGAGAGTCGAGATCGCCTTGGAGTACTTCTTTGCTTTGAGA
CTGCAACTTCTCCGAACAAGCTCACCGCAGCTTTCACCGATTGGAACGGTGCTCTTGTGT
CTATTTCTCCCACCTCCATCGTCCTCGGAGATTCCGGAGCTTTTTGTACTCTAATGTTCA
CCATTTTCTACTATCTTTTTTTTTTCAAAACCAACCTGCATACACAAACTTTC

Last Updated on 06.12.2018 16:11