Sequences from confirmation amplicon


Confirmation sequences for line 852D11 and hit At4g01110 are shown.
Line ID 852D11
Locus At4g01110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:480582
Primers used for PCR 29wu - 8474, show primer details
   
Sequence Name 50-K118874-0022-852-D11C-29wu
GenBank Accession LN507329 [GenBank]
Sequencing primer gene specific primer, 29wu: 5'-TTTATCCGACGAAGAAGAGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118874-0022-852-D11C-29wu
CGCCATTGTCATTCTCCTCCTTATCCTCACCGTCTCCGTTTTCTTTCTTTATTACAGCCC
GAGACTCCCCGTCGTACGTCTCTCCTCTTTCCGCGTCAGCAATTTCAACTTTTCCGGTGG
GAAAGCCGGCGACGGTTTATCGCAGTTGACGGCGGAGGCTACAGCGAGGCTTGATTTCAG
AAACCCTAACGGGAAGTTACGTTACTATTACGGTAACGTTGACGTGGCGGTGAGTGTAGG
AGAAGATGATTTCGAAACGATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K118873-0022-852-D11C-8409
GenBank Accession LN490886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118873-0022-852-D11C-8409
TTACATTGAATATATACTCATCGTTTCGAAATCATCTTCTCCTACACTCACCGCCACGTC
AACGTTACCGTAATAGTAACGTAACTTCCCGTTAGGGTTTCTGAAATCAAGCCTCGCTGT
AGCCTCCGCCGTCAACTGCGATAAACCGTCGCCGGCTTTCCCACCGGAAAAGTTGAAATT
GCTGACGCGGAAAGAGGAGAGACGTACGACGGGGAGTCTCGGGCTGTAATAAAGAAAGAA
AACGGAGACGGTGAGGATAAGGAGGAGAATGACAATGGCGACGGTGATGCAGACGCAGCA
GCAAAAGACGCGGCAACGACTCCATTGTCTCTTCTTCGATCCGGATAA

Last Updated on 06.12.2018 16:11