Sequences from confirmation amplicon


Confirmation sequences for line 852G02 and hit At1g68510 are shown.
Line ID 852G02
Locus At1g68510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25706709
Primers used for PCR AH65 - 8474, show primer details
   
Sequence Name 48-K105566-0022-852-G02C-AH65
GenBank Accession LN506507 [GenBank]
Sequencing primer gene specific primer, AH65: 5'-TTCAATGGACCGTTGTGTAGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K105566-0022-852-G02C-AH65
GGACGAAGTGATCAGGACCGGATTCGATGAGGTTAAGAAGACCGGCTCGACCGTAGAACT
TAGCAAGGAAGAGTGTAGCATTGGCTTGAGAGTCTGCGGATTTGATCCATTGAAGGCATG
GTCGAATGGTGCAATCTTGGTTGCAACCTTTGCGTAGAACCCTACACCCGTTGCAGCTGA
TTCTCATATTCCTTTATATGTTTATTAGTGACAAATATACGTATGATGTGTGTTTGTGTT
CGGAAATGGAACTCAAGGAAGTTGTGAACAATTTGAATTTATAAGGAAGAAGTTACTCAT
GAGTCATAAGTGAATGAGCTGGTATTTTTCAACTTTTGTGTGTTCTTGGAGAAAGTGGTG
GAAAAAAACTGGTGCAGTCCAGGCTTTTATAAAACAAATACATTAAAGATAATTACTTTC
TCAAGAGAGAAATTAGTAAAATATTTGTATAGATTTCTCAAAAGATAGAGAAAAGGCCAA
GAAATGAGGGGAAGGAGGTTGTGTGTTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 80-K105567w-0022-852-G02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K105567w-0022-852-G02C-8409
TTACAATTGAATATATCCTGAAACACACAACCTCCTTCCCCTCATTTCTTGGCCTTTTCT
CTATCTTTTGAGAAATCTATACAAATATTTTACTAATTTCTCTCTTGAGAAAGTAATTAT
CTTTAATGTATTTGTTTTATAAAAGCCTGGACTGCACCAGTTTTTTTCCACCACTTTCTC
CAAGAACACACAAAAGTTGAAAAATACCAGCTCATTCACTTATGACTCATGAGTAACTTC
TTCCTTATAAATTCAAATTGTTCACAACTTCCTTGAGTTCCATTTCCGAACACAAACACA
CATCATACGTATATTTGTCACTAATAAACATATAAAGGAATATGAGAATCAGCTGCAACG
GGTGTAGGGTTCTACGCAAAGGTTGCAACCAAGATTGCACCATTCGACCATGCCTTCAAT
GGATCAAATCCGCAGACTCTCAAGCCAATGCTACACTCTTCCTTGCTAAGTTCTACGGTC
GAGCCGGTCTTCTTAACCTCATCGAATCCGGTCCTGATCACCTTCGTCCCGGTACTGAAT
TATCTCTTTTACCTTTTTTTTTACTAC

Last Updated on 06.12.2018 16:11