Sequences from confirmation amplicon


Confirmation sequences for line 853D02 and hit At1g76200 are shown.
Line ID 853D02
Locus At1g76200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28593122
Primers used for PCR DA53 - 8474, show primer details
   
Sequence Name 71-K107116-0022-853-D02C-8409
GenBank Accession LN512569 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K107116-0022-853-D02C-8409
TTACAATTGAATATATCAGTGATAAAGGGCCCAAAAAGGAAGAAAACATTGTGAAAGAAA
CAGATATAGAAATGGGCCTTTGGTCTTTAAATTGGGCCTAAGCCTTTTAATTGAGGCCTT
ACTAAAAGTGACGACTCACATGGTTGAATCAAATCTCAGGTAGTAAATTGTCAAAGCTGA
GGAGAAAGAAAGCTTTTTCGGTCTGAAATTGAAGTGCGAATCGGGGAATCTAGGGTTTCG
AGAGAGAGATAATGGGTGGAGGAGGACATGGCGGAGGTATAACTTACAAGGGAGTCACTG
TCCACACTCCCAAGACTTGGCACACCGTCACCGGAAAAGGCTTGTGCGCCGTTATGTGGT
ACATTTCTGATTTCTCTCTTTCACCTAATTCGCTTTTGATTTTGGGACCTAGCTAATCTC
GATCGTAGCTAGATCTCGCCATGGGGATTTTGGGCTAATTGATCATCTGAGGAATTTTGT
ATATAGAATCCATTTGAATCAAAAAATTGGATTTGTCAAATTAGTATCGGAGCTAATCAG
GTAGATGTTAATTTGAATCAGATATTGCTTATGTCAATTTCAGTTCGATGAGAATGGTTG
AAAANTTAATCG
   
Sequence Name 87-K107117-0022-853-D02C-DA53
GenBank Accession LN514769 [GenBank]
Sequencing primer gene specific primer, DA53: 5'-GCGATTACTTCTTCAACCATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K107117-0022-853-D02C-DA53
AGCATATCTGATTCAATTAACATCTACCTGATTAGCTCCGATACTAATTTGACAAATCCA
ATTTTTTGATTCAAATGGATTCTATATACAAAATTCCTCAGATGATCAATTAGCCCAAAA
TCCCCATGGCGAGATCTAGCTACGATCGAGATTAGCTAGGTCCCAAAATCAAAAGCGAAT
TAGGTGAAAGAGAGAAATCAGAAATGTACCACATAACGGCGCACAAGCCTTTTCCGGTGA
CGGTGTGCCAAGTCTTGGGAGTGTGGACAGTGACTCCCTTGTAAGTTATACCTCCGCCAT
GTCCTCCTCCACCCATTATCTCTCTCTCGAAACCCTAGATTCCCCGATTCGCACTTCAAT
TTCAGACCGAAAAAGCTTTCTTTCTCCTCAGCTTTGACAATTTACTACCTGAGATTTGAT
TCAACCATGTGAGTCGTCACTTTTAGTAAGGCCTCAATTAAAAGGCTTAGGCCCAATTTA
AAGACCAAAGGCCCATTTCTATATCTGTTTCTTTCACAATGTTTTCTTCCTTTTTGGGCC
CTTTATCACTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11