Sequences from confirmation amplicon


Confirmation sequences for line 853D02 and hit At2g35710 are shown.
Line ID 853D02
Locus At2g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15014048
Primers used for PCR 33iv - 8474, show primer details
   
Sequence Name 51-K112877-0022-853-D02C-8409
GenBank Accession LN507557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112877-0022-853-D02C-8409
TCATTGAATATTCCTGTTCTTGTCTTTGGTTCTATCTTTTATGATAATCGAAACGACGGC
GTATCGAGAGAGACAGCTGCTGCTGCTGCAACCACCGCAAGAAACGGCGATAGATACCGC
AAACGCGGTGGTGACGGTTCAAGATCGAGGTTTGAAGACGCGGCGACCGGAGCATAAGAA
CGCATACGCAACGATGATGTACATGGGGACGCCAAGAGACTACGAGTTCTACGTTGCGAC
ACGTGTTTTGATCAGATCGTTGAGAAGTCTTCACGTGGAAGCTGATCTCGTCGTCATCGC
TTCTCTCGACGTTCCTCTCCGATGGGTTCAAACCTGGTAAGTCTC
   
Sequence Name 88-K112878-0022-853-D02C-33iv
GenBank Accession LN514880 [GenBank]
Sequencing primer gene specific primer, 33iv: 5'-AGAGACTTACAAGGTTTGAACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K112878-0022-853-D02C-33iv
CTTCACGTGAAGACTTCTCAACGATCTGATCAAAACACGTGTCGCAACGTAGAACTCGTA
GTCTCTTGGCGTCCCCATGTACATCATCGTTGCGTATGCGTTCTTATGCTCCGGTCGCCG
CGTCTTCAAACCTCGATCTTGAACCGTCACCACCGCGTTTGCGGTATCTATCGCCGTTTC
TTGCGGTGGTTGCAGCAGCAGCAGCTGTCTCTCTCGATACGCCGTCGTTTCGATTATCAT
AAAAGATAGAACCAAAGACAAGAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTATA

Last Updated on 06.12.2018 16:11