Sequences from confirmation amplicon


Confirmation sequences for line 854G03 and hit At4g29410 are shown.
Line ID 854G03
Locus At4g29410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14469647
Primers used for PCR 25dr - 8474, show primer details
   
Sequence Name 27-K110307-0022-854-G03C-25dr
GenBank Accession LN498437 [GenBank]
Sequencing primer gene specific primer, 25dr: 5'-ACTTATAAACATTGCCTCCGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110307-0022-854-G03C-25dr
CGCATCGACATAAATCATAACTTAACTCCGGCACGAGTCTCGTTCTCTTCTCTTTACCGC
CGTCATCTAGGTCTGCTAATATCAAGGTGAGCTTGAATCATCTCTTTACAAACCAATTTG
CCGTTCTTTGTTCTTCAATCTGTGTAGCCCTTGTTTTACCGCTTCATATATGTTGTGTTA
CTAAATGCTTTCTTCTTCAATAATCAGTGAAGGAGCTCGAAATGGCCACAGTTCCAGGAC
AGTTGATCTGGGAGATCGTGAAGAGAAACAACTGTTTCTTGGTGAAGCAATTCGGTAGAG
GTAATGCCAAGGTTCAGTTCAGCAAAGAGAGCAACAACCTCGTCAACATCAACTCGTACA
AGCACTCTGGTAATACACAACTGTTGCTTACTCTAGATTTATCTCAAGTTTGTTCTTTTG
TTGAATCTGTTGATATGAACACAAGTCATTACCTTTATATGTCTAGCTAAATGTAATTGG
GAAAATTAACAACTTGTTGAATTGATGACTATCTTTTTGTTGGTTACATGCTATTGGGTC
ATGTCCTAATAGGAAGCAAGTAAATGGCTTCAATTGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 11-K110306-0022-854-G03C-8409
GenBank Accession LN491165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K110306-0022-854-G03C-8409
GGAATTGAGCCATTTACTTGCTTCCTATTAGGACATGACCCAATAGCATGTAACCAACAA
AAAGATAGTCATCAATTCAACAAGTTGTTAATTTTCCCAATTACATTTAGCTAGACATAT
AAAGGTAATGACTTGTGTTCATATCAACAGATTCAACAAAAGAACAAACTTGAGATAAAT
CTAGAGTAAGCAACAGTTGTGTATTACCAGAGTGCTTGTACGAGTTGATGTTGACGAGGT
TGTTGCTCTCTTTGCTGAACTGAACCTTGGCATTACCTCTACCGAATTGCTTCACCAAGA
AACAGTTGTTTCTCTTCACGATCTCCCAGATCAACTGTCCTGGAACTGTGGCCATTTCGA
GCTCCTTCACTGATTATTGAAGAAGAAAGCATTTAGTAACACAACATATATGAAGCGGTA
AAACAAGGGCTACACAGATTGAAGAACAAAGAACGGCAAATTGGTTTGTAAAGAGATGAT
TCAAGCTCACCTTGATATTAGCAGACCTAGATGACGGCGGTAAAGAGAAGAGAACGAGAC
TCGTGCCGGAGTTAAGTTATGATTTATGTCGATGCGTTAATATATATACATTCTCTGCAA
AACCCTAGTTTGGTTCTCGGAGGCAATGTTTATAA

Last Updated on 06.12.2018 16:11