Sequences from confirmation amplicon


Confirmation sequences for line 855D07 and hit At2g20340 are shown.
Line ID 855D07
Locus At2g20340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8782344
Primers used for PCR IF36 - 8474, show primer details
   
Sequence Name 90-K101387-0022-855-D07C-8409
GenBank Accession LN515025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K101387-0022-855-D07C-8409
GCCTTTCATTGAATATATCCTGCTTCTTGTTCTTGTTCTTGTTCTTGGCTTCTCTGCGGG
ATAATGTTGTTAAATCAATATATATGTATTGGCTTTGCGTGGGAAACAGGCTTTATCGGG
GAAAATCGTACTACGTTGCGCAATAGGAGCGCCATTGACGGAGGAGAAGCACGTTAAGGA
GGCGTGGAAGATTATTCAGGAAGAAGCATCTTACTTGCTTCACAAGTAAATATGATAAAA
CAGTAAAAGAATAAAACGGTTTTTGCAACAAAACGAAGCACTTCGGTTTGGGTTGGGTTT
ATTTTGATATATAATAATAAACAGAAA
   
Sequence Name 90-K101393-0022-855-D07C-IF36
GenBank Accession LN515026 [GenBank]
Sequencing primer gene specific primer, IF36: 5'-TTAACCACAAAATAAACCCAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K101393-0022-855-D07C-IF36
AAAAAAATAGAGGATAAAATACCCCCAAGAAAGATCACCTTACTAAAGTGTACCGAAATC
AAACCGAAGTTGATAAGTCTTACTTAAGCAATTTTCGGTTTTATTATTATATTATCAAAT
TAAACCAAACCAAACCGAAGTGCTTCGTTTTGTTGGCAAAAACCGTTTTATTCTTTTACG
GTTTTATCAAATTTACTTGTGAAGCAAGTAAAATGCTTCTTCCTGAATAATCTTCCACGC
CTCCTTAACGTGCTTCTCCTCCGTCAATGGCGCTCCTATTGCGCAACGTAGTACGATTTT
CCCCGATAAAGCCTGTTTCCCACGCAAGCCAATACATA

Last Updated on 06.12.2018 16:11