Sequences from confirmation amplicon


Confirmation sequences for line 856H01 and hit At1g15330 are shown.
Line ID 856H01
Locus At1g15330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5274658
Primers used for PCR 64by - 8474, show primer details
   
Sequence Name 47-K108442-0022-856-H01C-64by
GenBank Accession LN506217 [GenBank]
Sequencing primer gene specific primer, 64by: 5'-CATTAAACATACTACTGGGCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K108442-0022-856-H01C-64by
TCATTAGCAAGTATCGATTGCCACGTGCTTCTAGATCATCATCACACGGCGTCCTGATCC
ATTCACATCAAATTTCGAGCCAAGATCGACACTTAAAAAGGTTCTCAAAGCAACAAGTTC
AAGCTACGACAACAGTAGTAGAACACTATGCAAGAAGAGAAGAGCAAAGAAGATCACAGC
CGTCTGATCAATGTCACGGCCAAAGATCTAACGGTCAGAAACCGACGGCTCGTCGAGGTA
CCATACACGGCTACACTTTCCCACGCAATGAACACATTGGTGGCTAACTCCATCTCAGCC
CTCCCAGTGGCTGCACCTCCAGGCCACTGGATCGGAGCCGGTGGTTCCATGATCATGGAA
TCAGACAAACAAACTGGAGTTGTAAGAAAACACTATATCGGGATTCTCACGATGCTTGAC
ATACTTGCTCATATCGCCAATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCATTATTA
   
Sequence Name 35-K108441-0022-856-H01C-8409
GenBank Accession LN501859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K108441-0022-856-H01C-8409
GCCATTTACTGTAGATTGGCGATATGAGCAAGTATGTCAAGCATCGTGAGAATCCCGATA
TAGTGTTTTCTTACACTCCAGTTTGTTTGTCTGATTCCATGATCATGGAACCACCGGCTC
CGATCCAGTGGCCTGGAGGTGCAGCCACTGGGAGGGCTGAGATGGAGTTAGCCACCAATG
TGTTCATTGCGTGGGAAAGTGTAGCCGTGTATGGTACCTCGACGAGCCGTCGGTTTCTGA
CCGTTAGATCTTTGGCCGTGACATTGATCAGACGGCTGTGATCTTCTTTGCTCTTCTCTT
CTTGCATAGTGTTCTACTACTGTTGTCGTAGCTTGAACTTGTTGCTTTGAGAACCTTTTT
AAGTGTCGATCTTGGCTCGAAATTTGATGTGAATGGATCAGGACGCCGTGTGATGATGAT
CTAGAAGCACGTGGCAATCGATACTTGCTAATGACATGCAACACGTGGCATTTTTCATTG
GGCCTTAGCTAACGGCCCAGTAGTATGTTTAATG

Last Updated on 06.12.2018 16:11