Sequences from confirmation amplicon


Confirmation sequences for line 857A03 and hit At5g04047 are shown.
Line ID 857A03
Locus At5g04047 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1095407
Primers used for PCR 82ra - 8474, show primer details
   
Sequence Name 57-K116162-0022-857-A03C-82ra
GenBank Accession LN509513 [GenBank]
Sequencing primer gene specific primer, 82ra: 5'-TGATATTGGTCGAATCCCATTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K116162-0022-857-A03C-82ra
ATTTCGTACATAAATTCCATCTTCTTGCGCTTGCCAGAAACATATGTTAGGTTTTCTCCC
TCCACATATATCATGTATAAAACTTTCTTTCCCAATAAATGCGTTAAACTTCGCATGATG
CACATAGTTAGGTCCCTGAAAAAATATATGTATTTCAAATATATAAAACTTGAGACTATC
GTAGTAATGAATATGTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 28-K116161-0022-857-A03C-8409
GenBank Accession LN498993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116161-0022-857-A03C-8409
CATTACATTACATATTCATTACTACGATAGTCTCAAGTTTTATATATTTGAAATACATAT
ATTTTTTCAGGGACCTAACTATGTGCATCATGCGAAGTTTAACGCATTTATTGGGAAAGA
AAGTTTTATACATGATATATGTGGAGGGAGAAAACCTAACATATGTTTCTGGCAAGCGCA
AGAAGATGGAATTTATGTACGAAATAATGCAACTGGAACCTTTAAGTTAATGTATAAATG
GGATTCAACCAATATCAA

Last Updated on 06.12.2018 16:11