Sequences from confirmation amplicon


Confirmation sequences for line 857A11 and hit At3g44428 are shown.
Line ID 857A11
Locus At3g44428 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16067469
Primers used for PCR 32qp - 8474, show primer details
   
Sequence Name 82-K115779-0022-857-A11C-32qp
GenBank Accession LN514228 [GenBank]
Sequencing primer gene specific primer, 32qp: 5'-CCAAGAATCAAACTATGGGTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K115779-0022-857-A11C-32qp
ACCCTAGACCATGTTTAGTTAGTAGAGCCAACACATTATTTTGTACTTATTACAAAACAA
TTTAGGTAGTGATTAACAAATTACTAAAAGAACTATCATTTTATTAGAAATATTAATAAT
ACAAATTACCATTACATGAAAAAAAATATCTAAGGATTGAAAAAATTGTGATTAAAAAAT
GCCTATTAATTTGCATGTCATCTTAAGTATAATACGGTAGTAGAAACGCATAGGAAATAA
AATCCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCG
   
Sequence Name 34-K115778-0022-857-A11C-8409
GenBank Accession LN501542 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115778-0022-857-A11C-8409
TCGGACATGAGCCTTTACATTGGGATTTTATTTCCTATGCGTTTCTACTACCGTATTATA
CTTAAGATGACATGCAAATTAATAGGCATTTTTTAATCACAATTTTTTCAATCCTTAGAT
ATTTTTTTTCATGTAATGGTAATTTGTATTATTAATATTTCTAATAAAATGATAGTTCTT
TTAGTAATTTGTTAATCACTACCTAAATTGTTTTGTAATAAGTACAAAATAATGTGTTGG
CTCTACTAACTAAACATTGGTCTAGTGGTTGTGAGACTCTTTGTTGGGAGCGAGAAGATT
GACCCATAGTTTGATTCTTGG

Last Updated on 06.12.2018 16:11