Sequences from confirmation amplicon


Confirmation sequences for line 859B10 and hit At4g35810 are shown.
Line ID 859B10
Locus At4g35810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16969154
Primers used for PCR NR80 - 8474, show primer details
   
Sequence Name 70-K102596-0022-859-B10C-8409
GenBank Accession LN512362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102596-0022-859-B10C-8409
TTTACAGATCAAGCAAAAACACAAAACATCAGAGAATCATATCCCTAACCTCTCCTGGAT
CGTTTGTACAATGGTGGTCAAATCCATCGGCATAGAAGAGGTCTTGTTGGTGCTGGGTAG
TGAAAAGATTCCGAGACCGACAAGGATCAAAATGACAAAGAGTACAAGTACGACAACTGT
GAAAGTTTGCGTCGAAAAAGATTTACGCGGCTTGTTACGGAGCTGCTTCGGTTTCTTCGC
CATTTTTGTTTGATGATCTAGATAGTTTGAAGAATCCTATTGAGCGATCTTAATCAAAAG
AACAAAAGAAGTTATAACGAAAACATGCATTTGAAGATAAAAGCTATAAAGTATAAATAA
TTTTCTTTCTTTTTTCGTTGTTTTGTTTTGGTAATCA
   
Sequence Name 70-K102597-0022-859-B10C-NR80
GenBank Accession LN512363 [GenBank]
Sequencing primer gene specific primer, NR80: 5'-GCTGATACAAAAACAAAACAACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102597-0022-859-B10C-NR80
CTTTAAGCTTTATCTTCAATGCATGTTTTCGTTATAACTTCTTTTGTTCTTTTGATTAAG
ATCGCTCAATAGGATTCTTCAAACTATCTAGATCATCAAACAAAAATGGCGAAGAAACCG
AAGCAGCTCCGTAACAAGCCGCGTAAATCTTTTTCGACGCAAACTTTCACAGTTGTCGTA
CTTGTACTCTTTGTCATTTTGATCCTTGTCGGTCTCGGAATCTTTTCACTACCCAGCACC
AACAAGACCTCTTCTATGCCGATGGATTTGACCACCATTGTACAAACGATCCAGGAGAGG
TTAGGGATATGATTCTCTGATGTTTTGTGTTTTTGCTTGATCTTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATG

Last Updated on 06.12.2018 16:11