Sequences from confirmation amplicon


Confirmation sequences for line 859E04 and hit At5g20885 are shown.
Line ID 859E04
Locus At5g20885 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7084147
Primers used for PCR MC65 - 8474, show primer details
   
Sequence Name 31-K035355-022-859-E04C-8409
GenBank Accession LN500066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K035355-022-859-E04C-8409
ATTCCGGACTGAGCCATTTAAATTGATCCATCTCCGAAGAGGTAGAGAAGCCGTTCAACG
GCCCAGCTAGGTTCGTTCTTCGTCGGCCAATCACCACACGACGTCGTATTAGCAGCAAGT
AGCGGAGTTCTACAAAGAGGACACGTACGGTGATTATCCTCCTCACCTTCGTTATTCTCA
TCACCACCGCAACATTCATAATCCAACCACCGGTCAATACACTCACGGTGAAACATATGG
CTACAGTTCCTGAGTTCTCTAACCTCATCTCCATCTTCCAGATCTCCGAGACACACAGCG
CAGGTGTCGTTGATCATCGCCGGAGATCTCTCAGCTGCGTCACGGAACGTCGTCACGGAG
AGGCTTTCTTTGATCGCCTGTGAGGAAATCGAAGGACAGGCGGAAGTGGAAGAAGAGGTG
GATCTGGATCTGGATCGGTAGCGTAAGATCCAAGCGAGGATCCATCTCAAGACGGTGATG
AGAACAGCCATTTTGTAGAGAAGCTGAGTGACTATGAGACCAGAATCTTCGATGAAGAAG
CTCATTGTGATGACTTTGCTCAAGTATGTGCGTTTGTGTGTGTGTGTGAGAGAGAGAGAG
AGAGAGAGAAGGGGAGGAGAAAGTGGAGAAT
   
Sequence Name 35-K035355-022-859-E04C-MC65
GenBank Accession LN501736 [GenBank]
Sequencing primer gene specific primer, MC65: 5'-AAACGAATCTCATTCTCCACTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K035355-022-859-E04C-MC65
CTCTTCTCTCTCACACACACACACAAACGCACATACTTGAGCAAAGTCATCACAGTGTTC
TTCTTCATCGAAGATTCTGGTCTCATAGTCACTCATCTTCTCTACAAAATGGCTGTTCTC
ATCACCGTCTTGAGATGGATCCTCGCTTGGATCTTACGCTACCGATCCAGATCCAGATCC
ACCTCTTCTTCCACTTCCGCCTGTCCTTCGATTTCCTCACAGGCGATCAAAGAAAGCCTC
TCCGTGACGACTTTCCGTGACGCATCTGAGAGATCTCCGGCGATGATCAACGACACCTGC
GCTGTGTGTCTCGGAGATCTGGAAGATGGAGATGAGGTTAGAGAACTCAAGAACTGTAGC
CATATGTTTCACCGTGAGTGTATTGACCGGTGGTTGGATTATGAATGTTGCGGTGGTGAT
GAGAATAACGAAGGTGAGGAGGATAATCACCGTACGTGTCCTCTTTGTAGAACTCCGCTA
CTTGCTGCTAATACGACCTCGTGTGGTGATTGGCCGACAAAGAACGAACCTAGCTGGGCC
GTTGAACGGCTTCTCTACCTCTTCAGAGATGGATCATTTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAGTGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCATTTTTTTTTCAATTCAAAAATGTATATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACTATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCAGATATCTTTATCTCGACGTGTCTACTTCACGTCCCAAGTGGGGAGGG
GG

Last Updated on 06.12.2018 16:11