Sequences from confirmation amplicon


Confirmation sequences for line 859H01 and hit At3g01328 are shown.
Line ID 859H01
Locus At3g01328 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:117388
Primers used for PCR 43wc - 8474, show primer details
   
Sequence Name 89-K118625-0022-859-H01C-43wc
GenBank Accession LN514985 [GenBank]
Sequencing primer gene specific primer, 43wc: 5'-TGTTTCATATGTAAAAGGATCCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K118625-0022-859-H01C-43wc
CTTTAAATCTTCGAATATAGATGTTATATCAGTTAAATCCTTTTATATCCAGTATAAATC
TTTGATATATTTGTTGAATCCAACAAAACTAAAACTCCATTGGTTAAACTAATTATGTTT
AAAAAAGTATTGCTTGGTCAAAACTCCATAAATTGATTGGGTCAAAAAAAAATTACGTGG
TCTTTCTTTAGTCACTCTTCTTATAGAAGGAGATCCTTTTCTTTTGGGAAGGAAATCCTT
AGAATACATTCCACACCAAAAAAAACTTCACATCATTTCTCTACAACTAACTACACAAAC
TTTATAAAGTATATAAACCATATATTTTCCGTCTTCTGTTTATTAAAAAAGAAAACAGAA
AATGAAACCAAAGCAAATCAAAATGATGTTCTTTTTATTAATTGTAGTTGCAGCTATAAT
TTTCCGACCATCCAAAGCTCAGCTAAAAACGAGCATATGTACAAGTAAACAGACAACGCC
AATTACACAGGTGGCTGGATGTTTCAATGCGGTGAGATTAGCTGCGGACAAAGATTCCAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 86-K118624-0022-859-H01C-8409
GenBank Accession LN514700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K118624-0022-859-H01C-8409
GCCATTTGGAATCTTTGTCCGCAGCTAATCTCACCGCATTGAAACATCCAGCCACCTGTG
TAATTGGCGTTGTCTGTTTACTTGTACATATGCTCGTTTTTAGCTGAGCTTCGGATGGTC
GGAAAATCATAGCTGCAACTACAATTAATAAAAAGAACATCATTTTGATTTGCTTTGGTT
TCATTTTCTGTTTTCTTTTTTAATAAACAGAAGACGGAAAATATATGGTTTATATACTTT
ATAAAGTTTGTGTAGTTAGTTGTAGAGAAATGATGTGAAGTTTTTTTTGGTGTGGAATGT
ATTCTAAGGATTTCCTTCCCAAAAGAAAAGGATCTCCTTCTATAAGAAGAGTGACTAAAG
AAAGACCACGTAATTTTTTTTTGACCCAATCAATTTATGGAGTTTTGACCAAGCAATACT
TTTTTAAACATAATTAGTTTAACCAATGGAGTTTTAGTTTTGTTGGATTCAACAAATATA
TCAAAGATTTATACTGGATATAAAAGGATTTAACTGATATAACATCTATTTTTCGAAGAT
TTAAAGTGATCTAACAAACGAGATCCTGTTTTTCTTGGTTCGGGGATCCTTTTACATATA
AAACA

Last Updated on 06.12.2018 16:11