Sequences from confirmation amplicon


Confirmation sequences for line 859H10 and hit At5g63660 are shown.
Line ID 859H10
Locus At5g63660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25485826
Primers used for PCR CP08 - 8474, show primer details
   
Sequence Name 24-K031008-022-859-H10C-8409
GenBank Accession LN496882 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K031008-022-859-H10C-8409
CCATTTACAATTGAAATATTACATATATCATATATATATATATTTATTGTCAATCAAATA
TGGTCATGGACCAATGCATATATATAGAAGGAAAAGACTTACGAGATGAGAAGAGGACAA
GAAGCAAGAAGAATGCAGCGAAAAACTTGTTCTCCATATATAATCCTAATGATATTGATT
GCTTGTTAAATGCTAAAAACGTAAGTGGAGCAAGAGCTACTTCTCCATGACTCCATATAT
ATAGATTCAAGTGTAGGTTTCGTTAGGGTTTCTTTTTGGGGCCACATAGTGTACTCAACT
AAACATCGAGTTTTAAACAATTTATTAGTATTAAAAAAAAAAAAATGCTATGGTAAGAGC
TAGTCTCCATCGTATTTTATTAGTTAAATGCTATGGTCAAGGATGTGTGGATAAAGTATA
AACGATGAATTGCCCCACACACAATAA
   
Sequence Name 25-K031008-022-859-H10C-CP08
GenBank Accession LN497319 [GenBank]
Sequencing primer gene specific primer, CP08: 5'-GTTTTATTGTGTGTGGTGCAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K031008-022-859-H10C-CP08
ATAGCATCTAACTAATAAAATACGAATGGAGACTCAGCTCTTACCATAGCATCTTTTTTT
TTTTAATACTAATAAATTGTTTAAAACTCGATGTTTAGTTGAGTACACTATGTGGCCCCA
AAAAGAAACCCTAACGAAACCTACACTTGAATCTATATATATGGAGTCATGGAAAAGTAG
CTCTTGCTCCACTTACGTTTTTAGCATTTAACAAGCAATCAATATCATTAGGATTATATA
TGGAGAACAAGTTTTTCGCTGCATTCTTCTTGCTTCTTGTCCTCTTCTCATCTCGTAAGT
CTTTTCCTTCTATATATATGCATTGGTCCATGACCATATTTGATTGACAATAAATATATA
TATATATGATATATGTAATATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAAATTTCCGCAGCGTTTTTATAAAATGAAAGTACTTTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATGGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACTTT

Last Updated on 06.12.2018 16:11