Sequences from confirmation amplicon


Confirmation sequences for line 861E05 and hit At3g16857 are shown.
Line ID 861E05
Locus At3g16857 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5755897
Primers used for PCR 27gd - 8474, show primer details
   
Sequence Name 65-K112062-0022-861-E05C-27gd
GenBank Accession LN511431 [GenBank]
Sequencing primer gene specific primer, 27gd: 5'-TTTCATTGATGATTCAACCCCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K112062-0022-861-E05C-27gd
CAATACCCAGAATTTGAGCTCAAGCACATCCAAAATGTTCTTCTAAATAGAAAAATAAAA
CAGAATATAGAACAATAGTACATATAATATACAAAACTAACTTGTAAGAAATTGAGAATA
AAAAAGAGAGTGGATCTAAATGAAATTTAATAAAACCAATAGTCTAAATGTAGAGAGAAT
GATGAAAATAAAGGACATGTCAGCTTCTACATATATGTAAATAAAAAATATATATGACTG
AGTATATAAAAACCACATTTTTTTTTTCTTCTGCATCTAAATGTTACAAAAATTGAATAG
GAAAAATTAAAAGAGCTTTACTCTGTGAATAAATAAAAAAACAGAGAAAAAGAGCTCTAA
GGTGAGACAAGCCTTGTCTTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAAATTTCCGC
   
Sequence Name 49-K112045-0022-861-E05C-8409
GenBank Accession LN506886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112045-0022-861-E05C-8409
GCATTTACATTGAATAATCCTGAAGAAGACAGGCTTGTCTCACCTTAGAGCTCTTTTTCT
CTGTTTTTTTATTTATTCACAGAGTAAAGCTCTCTTAATTTTTCCTATTCAATTTTTGTA
ACATTTAGATGCAGAAGAAAAAAAAAATGTGGTTTTTATATACTCAGTCATATATATTTT
TTATTTACATATATGTAGAAGCTGACATGTCCTTTATTTTCATCATTCTCTCTACATTTA
GACTATGGGTTTTATTAAATTTCATTTAAATCCACTCTCTTTTTTATTCTCAATTTCTTA
CAAGTTAGTTTTGTATATAATATGTACTATTGTTCTATATTCTGTTTTATTTTTCTATTT
AGAAAAACATTTTGGATGTTGCTTTGAGCTCAAATTCTGGGTTATTTTGTGAAGTAGTGA
AATATAGGGGTTGAATCATCAATGAA

Last Updated on 06.12.2018 16:11