Genotyping and primer details


Genotyping and primer details for 861E05 - At3g16857
Line ID 861E05
Locus At3g16857 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5755897 of line 861E05 Chr3:5755897
   
Insertion allele amplicon 8474 - 27gd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27gd: 5'-TTTCATTGATGATTCAACCCCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5755470
   
wt amplicon (over InsPos) 01hn - 27gd
wt amplicon start Chr3:5755470
wt amplicon end Chr3:5756325
wt amplicon size 856 bp
Locus specific primer 27gd: 5'-TTTCATTGATGATTCAACCCCTAT-3'
2nd locus specific primer 01hn: 5'-GAAGATCTTGGTTTTTGGATACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11