Sequences from confirmation amplicon


Confirmation sequences for line 861F11 and hit At3g53570 are shown.
Line ID 861F11
Locus At3g53570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19863695
Primers used for PCR FV45 - 8474, show primer details
   
Sequence Name 68-K033879-022-861-F11C-FV45
GenBank Accession LN511946 [GenBank]
Sequencing primer gene specific primer, FV45: 5'-ACGATAGTCAAACCAATTCCGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K033879-022-861-F11C-FV45
GAAAGCTTATACCAAATGTAATAAAATTAAAACTNAGCGAAGAAATAGTAAGAGGAAAGA
AATATTCTTACCGAGAACCACCGACATCATGCCTTGTAAGCCTCTGCAGCACGTCAATTT
CGATCATGGCAGCTTCACGATACTTGTTTATGGACCGTATAACTTTGATTGCCACGACTT
CTTTGTTTTTATTATCAAAGCATTCCAATACTTGTCCAAATGTACCTGCCAAAATACAAT
CCAATTGATTAAGATAACTGGCGACCAAATAAACATATTTCTCCTACACTTTGAAAAACA
GAATAATCTAATCATAATGATTATATAAACATTTTCGCAAGAGATGACTAACCTTCACCC
ATTTTGCTGAGAATTTGATCTGCACGCACAATAGAAGAGGCATGTCAATGTAAACCTCCA
TGATAAAGTCTGTGAAACGTAACAGATAAGATGTCGAAAGACTCTGTAACATAAGACTCA
CATCGTGGAGTCAAAGTATCCCCAACAACAAAGACATAATGGCCGTCTTTATCATCTGGT
CTCCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 12-K033879-022-861-F11C-8409
GenBank Accession LN491474 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K033879-022-861-F11C-8409
TGGAGACCAGATGATAAAGACGGCCATTATGTCTTTGTTGTTGGGGATACTTTGACTCCA
CGATGTGAGTCTTATGTTACAGAGTCTTTCGACATCTTATCTGTTACGTTTCACAGACTT
TATCATGGAGGTTTACATTGACATGCCTCTTCTATTGTGCGTGCAGATCAAATTCTCAGC
AAAATGGGTGAAGGTTAGTCATCTCTTGCGAAAATGTTTATATAATCATTATGATTAGAT
TATTCTGTTTTTCAAAGTGTAGGAGAAATATGTTTATTTGGTCGCCAGTTATCTTAATCA
ATTGGATTGTATTTTGGCAGGTACATTTGGACAAGTATTGGAATGCTTTGATAATAAAAA
CAAAGAAGTCGTGGCAATCAAAGTTATACGGTCCATAAACAAGTATCGTGAAGCTGCCAT
GATCGAAATTGACGTGCTGCAGAGGCTTACAAGGCATGATGTCGGTGGTTCTCGGTAAGA
ATATTTCTTTCCTCTTACTATTTCTTCGCTAGTTTTAATTTTATTACATTTGGTATAAGC
TTTTCAATTCCTTGCAGTTGTGTGCAAATACGGAATTGGTTTGACTATCG

Last Updated on 06.12.2018 16:11