Sequences from confirmation amplicon


Confirmation sequences for line 861H03 and hit At1g23240 are shown.
Line ID 861H03
Locus At1g23240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8253350
Primers used for PCR DA58 - 8474, show primer details
   
Sequence Name 92-K107706-0022-861-H03C-DA58
GenBank Accession LN515197 [GenBank]
Sequencing primer gene specific primer, DA58: 5'-AGCAAGAAGGAAATGTGAATGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K107706-0022-861-H03C-DA58
GTTTCAGGTTAATTATGCAACAATCTATATATATATATATGTTAAACTATGCGGATATAT
TTTACCATTGTTTTTTGAAGCCATAGTGATTGGTGATTAGGGAAAGGGATTCTCGCCGTT
GTTTCCAATAGACGTGAAGAATAGTCACCTTTGCATGCACGGTAGTGACACCGACGTCTA
TGACGATGACGGAAGGTTTTTTTTCATCTCTTCCTTGGCATCTTTTTCTTTTGTTAATGT
AATAATGTACATGTGTGTGTTTTTGTACGTAATTACAAAATCCAACTTTACAAAAGTGTC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 76-K107705-0022-861-H03C-8409
GenBank Accession LN513409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K107705-0022-861-H03C-8409
GCCATTTACATTGAATTATCCTGACACTTTTGTAAAGTTGGATTTTGCCATTACGTACAA
AAACACACACATGTACATTATTACATTAACAAAAGAAAAAGATGCCAAGGAAGAGATGAA
AAAAAACCTTCCGTCATCGTCATAGACGTCGGTGTCACTACCGTGCATGCAAAGGTGACT
ATTCTTCACGTCTATTGGAAACAACGGCGAGAATCCCTTTCCCTAATCACCAATCACTAT
GGCTTCAAAAAACAATGGTAAAATATATCCGCATAGTTTAACATATATATATATATAGAT
TGTTTGCATAATTAAACCTAGAAACAAAAACTAATCAACCAACTAATTAAATACTCATTC
ACATTTCCTTCTTG

Last Updated on 06.12.2018 16:11