Sequences from confirmation amplicon


Confirmation sequences for line 863H08 and hit At3g55500 are shown.
Line ID 863H08
Locus At3g55500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20576011
Primers used for PCR KQ74 - 8474, show primer details
   
Sequence Name 02-K101499-0022-863-H08C-8409
GenBank Accession LN486966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101499-0022-863-H08C-8409
CATTTACAATTGAATATATCCTGAGAAGACTCTAGGGATTCCGGCGTCGGTGAAGCTCAA
GAGAAGGAAGAGTGGGAATATGGTTAAGAGAATCAATGGATTGATTGCCATTTTGGTAAG
TATTTTTAATTGAATTGGAGAAAGCTTGAGAAAATTGTTAGACTAGAAGGCTTAGAGAGG
AACTTGAGGATGGTGGAAAATACACTTGTGAAGATCGCTATTTATATTGGTGGAGAGAAC
TCCAAAGTTAGAGAAATAGATGGTACTGGCCGAAATTAGTATTTTTCTAGACAAATATTT
AGAAAGTAGATGGTGAAAATATAGGTTCATAACCGTTTGTGATCTTTTTATTATTATTGC
AATGAGATGAGATCTTTAATCCGATTAACTCTGGGATCTATTAATAATACAATGTGAGAT
TAACTTCAAATTAAAATAGCTNATTGGAAAATGTCCAACCA
   
Sequence Name 02-K101503-0022-863-H08C-KQ74
GenBank Accession LN486967 [GenBank]
Sequencing primer gene specific primer, KQ74: 5'-ATTTCTACGTGGTTGGACATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101503-0022-863-H08C-KQ74
TTATCTCACATTGTATTATAATAGATCCCAGAGTTAATCGGATTAAAGATCTCATCTCAT
TGCAATAATAATAAAAAGATCACAAACGGTTATGAACCTATATTTTCACCATCTACTTTC
TAAATATTTGTCTAGAAAAATACTAATTTCGGCCAGTACCATCTATTTCTCTAACTTTGG
AGTTCTCTCCACCAATATAAATAGCGATCTTCACAAGTGTATTTTCCACCATCCTCAAGT
TCCTCTCTAAGCCTTCTAGTCTAACAATTTTCTCAAGCTTTCTCCAATTCAATTAAAAAT
ACTTACCAAAATGGCAATCAATCCATTGATTCTCTTAACCATATTCCCACTCTTCCTTCT
CTTGAGCTTCACCGACGCCNGAATCCCTAGAGTCTTCTCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11