Sequences from confirmation amplicon


Confirmation sequences for line 864A05 and hit At5g35790 are shown.
Line ID 864A05
Locus At5g35790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13957384
Primers used for PCR SD17 - 8474, show primer details
   
Sequence Name 89-K103502-0022-864-A05C-SD17
GenBank Accession LN514946 [GenBank]
Sequencing primer gene specific primer, SD17: 5'-TGGATAGAGCTCAGGAATGATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K103502-0022-864-A05C-SD17
CAAGCCGAGTGTAGATAGATCCCATGCTGCATCTAGCTCATCACTTCTGATAAACAACCT
CCTCTCTCCTTCGATTGCATCGAGAAGCAGTCTTTCATATGCATCTGGTATCTCTCTTGG
ATACCTATCAACCCCAAAAGAATATAAATAAATAATCTCTATAGGAAAACTAGAATCAGT
AGTATGCATTGTTGTACCTGGATCGATATAGAAGGTTAAGGTCGCTGCGATCTAGTCTCA
TTCCAAGACCAGGAACTTTGTTGTTGATCCTCAGGTATATCCCTTCATCAGGCTGAACTC
TAATCACTAGTTCATTTGTTGCATTGTCTAAATTAGTAGCAAAACTTTTCTTGTACAAGT
TTCCTGGGACATGTCTGAGCTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAAAGTAGATATCCGC
   
Sequence Name 92-K103503-0022-864-A05C-8409
GenBank Accession LN515190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K103503-0022-864-A05C-8409
GCCATTTACAATTGAATATATCCTGTCAGCTCAGACATGTCCCAGGAAACTTGTACAAGA
AAAGTTTTGCTACTAATTTAGACAATGCAACAAATGAACTAGTGATTAGAGTTCAGCCTG
ATGAAGGGATATACCTGAGGATCAACAACAAAGTTCCTGGTCTTGGAATGAGACTAGATC
GCAGCGACCTTAACCTTCTATATCGATCCAGGTACAACAATGCATACTACTGATTCTAGT
TTTCCTATAGAGATTATTTATTTATATTCTTTTGGGGTTGATAGGTATCCAAGAGAGATA
CCAGATGCATATGAAAGACTGCTTCTCGATGCAATCGAAGGAGAGAGGAGGTTGTTTATC
AGAAGTGATGAGCTAGATGCAGCATGGGATCTATTCACACCGGCTTTGAAGGAACTAGAG
GAGAAGAAAATCATTCCTGACCTCTATCC

Last Updated on 06.12.2018 16:11