Sequences from confirmation amplicon


Confirmation sequences for line 864H02 and hit At4g37870 are shown.
Line ID 864H02
Locus At4g37870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17805895
Primers used for PCR 41vf - 8474, show primer details
   
Sequence Name 08-K118356-0022-864-H02C-41vf
GenBank Accession LN489928 [GenBank]
Sequencing primer gene specific primer, 41vf: 5'-TGATCTTCTCCTCCGATCATTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118356-0022-864-H02C-41vf
CTCTTCCTCCGGTAAATTTTTACGTCGATGGTAATTTTAATATCAGTACGAGTGTAATCA
ACTTCGTAACTCTGTCTTTTTTTTGTGTTGTGGTTACAGGAAAAAGATGTCGGCCGGTAA
CGGAAATGCTACTAACGGTGACGGAGGGTTTAGTTTCCCTAAAGGACCGGTGATGCCGAA
GATAACGACCGGAGCAGCAAAGAGAGGTAGCGGAGTCTGCCACGACGATAGTGGTCCGAC
GGTGAATGCCACAACCATCGATGAGCTTCATTCGTTACAGAAGAAACGTTCTGCTCCTAC
CACACCGATCAACCAAAACGCCGCCGCTGCTTTTGCCGCCGTCTCCGAGGAGGAGCGTCA
GAAGATTCAGCTTCAATCTATCAGGTCCTTATAATAACTTCACATATACAGATTATTCAT
ACGTTACTTTTGTTTATAACATACTTTATATCGAATTAAGGAAGATTATTGCGTTTTCGT
GTCCGATCATTTTCATGGAAAAAGTGTCTTTTAGCTAAATATATGGTGTAGTATTAAATA
TTTCATGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K118355-0022-864-H02C-8409
GenBank Accession LN489927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118355-0022-864-H02C-8409
TTACATTGAATATATCCTGCCATGAAATATTTAATACTACACCATATATTTAGCTAAAAG
ACACTTTTTCCATGAAAATGATCGGACACGAAAACGCAATAATCTTCCTTAATTCGATAT
AAAGTATGTTATAAACAAAAGTAACGTATGAATAATCTGTATATGTGAAGTTATTATAAG
GACCTGATAGATTGAAGCTGAATCTTCTGACGCTCCTCCTCGGAGACGGCGGCAAAAGCA
GCGGCGGCGTTTTGGTTGATCGGTGTGGTAGGAGCAGAACGTTTCTTCTGTAACGAATGA
AGCTCATCGATGGTTGTGGCATTCACCGTCGGACCACTATCGTCGTGGCAGACTCCGCTA
CCTCTCTTTGCTGCTCCGGTCGTTATCTTCGGCATCACCGGTCCTTTAGGGAAACTAAAC
CCTCCGTCACCGTTAGTAGCATTTCCGTTACCGGCCGACATCTTTTTCCTGTAACCACAA
CACAAAAAAAAGACAGAGTTACGAAGTTGATTACACTCGTACTGATATTAAAATTACCAT
CGACGTTAAAAATTTTACCGGAGGAAGAGAGATCACGAACAAGAAGAGGCTGAGAAAGAT
TAAAGAGACTAATGATCGGA

Last Updated on 06.12.2018 16:11