Sequences from confirmation amplicon


Confirmation sequences for line 866E05 and hit At5g46200 are shown.
Line ID 866E05
Locus At5g46200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18729358
Primers used for PCR 40sf - 8474, show primer details
   
Sequence Name 58-K116491-0022-866-E05C-40sf
GenBank Accession LN509804 [GenBank]
Sequencing primer gene specific primer, 40sf: 5'-CTTATTCATCCCCAACAAATTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116491-0022-866-E05C-40sf
TATCACTTTGACTAGGGCTGATTTTGTTTTACGCTTATATTGATTTCTTCGTCTAGTGTA
CATCTTCAATAAACATATACTTTGTAATTACTTTTATTTTTATAATTTGTAACGATGTTT
GATAAAAAGATCAATGTTAACAATATATAAAACTAAATATTTGAACTAGTAATTACGAAC
ACAAATATAACTATTTACACTTTTTTCTATTCTGAAATGGTTAGAAACTTAGAATTTCTG
TATTTGTTTTTAAATCTTTAAGATATATATATATATATTTTTTTTTAACTTTTACTACAA
TTTGTTTTCTCGTTTATTTCAGGCCGTATCCTGAAACAAAGTCGCAGGGATAAAAAGAAG
AAAAAGAACAATCGTAAATATGAGTATGAGTATGATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCA
   
Sequence Name 26-K116490-0022-866-E05C-8409
GenBank Accession LN498086 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116490-0022-866-E05C-8409
ATTGAATCATACTCATACTCATATTTACGATTGTTCTTTTTCTTCTTTTTATCCCTGCGA
CTTTGTTTCAGGATACGGCCTGAAATAAACGAGAAAACAAATTGTAGTAAAAGTTAAAAA
AAAATATATATATATATATCTTAAAGATTTAAAAACAAATACAGAAATTCTAAGTTTCTA
ACCATTTCAGAATAGAAAAAAGTGTAAATAGTTATATTTGTGTTCGTAATTACTAGTTCA
AATATTTAGTTTTATATATTGTTAACATTGATCTTTTTATCAAACATCGTTACAAATTAT
AAAAATAAAAGTAATTACAAAGTATATGTTTATTGAAGATGTACACTAGACGAAGAAATC
AATATAAGCGTAAAAACAAAATCAGCCCTATGTCAAAGTTGATTAAGACAAGAGTACTTA
CCTGCTCTATGAATTTGTTGGGGAAGGAATAAG

Last Updated on 06.12.2018 16:11