Sequences from confirmation amplicon


Confirmation sequences for line 869A08 and hit At2g45890 are shown.
Line ID 869A08
Locus At2g45890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18883680
Primers used for PCR MF27 - 8474, show primer details
   
Sequence Name 78-K031693-022-869-A08C-8409
GenBank Accession LN513627 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K031693-022-869-A08C-8409
GCCTTTACAATTGAATATATCCTGGAGACATCTTCCCACTAACGCTGTTGTCTTGAGTGG
CCATGAAGAAGATGTCTCCGAAGACGCCGAGGAACCAAAAGATGATGTCGTCAACGACGT
CCACGGAGATGGAGACGAAGAAGACAGCGACATTGATTCTGCAGAAGATGCAGGTAAAGT
GTTTAAGATTGTAACTTTCTTCTTTTGTCATTTCGTTAAATCGGGGTTTCTAGTGCAGAG
CTAGAGATGATGAGGGAAAGATTTGCGAAGCTGTTGCTTGGTGAAGACATGTCAGGAAGT
GGGAAAGGAGTTTGTACTGCTGTTACTGTGTCAAACTCCATAACCAATCTCTATGGTTTG
TCTTCTTCTTCTCTTTTCAAATATGCTCTGTTTTCAAAACAGAGTATTTATAGTGTAGCG
TGTGTTTGTTGTGTTATTGCAGCGACTGTGTTTGGACAGAGTTTA
   
Sequence Name 30-K031693-022-869-A08C-MF27
GenBank Accession LN499603 [GenBank]
Sequencing primer gene specific primer, MF27: 5'-TTAAACTCTGTCCAAACACAGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K031693-022-869-A08C-MF27
CACTATAATACTCTGTTTTGAAAACAGAGCATATTTGAAAAGAGAAGAAGAAGACAAACC
ATAGAGATTGGTTATGGAGTTTGACACAGTAACAGCAGTACAAACTCCTTTCCCACTTCC
TGACATGTCTTCACCAAGCAACAGCTTCGCAAATCTTTCCCTCATCATCTCTAGCTCTGC
ACTAGAAACCCCGATTTAACGAAATGACAAAAGAAGAAAGTTACAATCTTAAACACTTTA
CCTGCATCTTCTGCAGAATCAATGTCGCTGTCTTCTTCGTCTCCATCTCCGTGGACGTCG
TTGACGACATCATCTTTTGGTTCCTCGGCGTCTTCGGAGACATCTTCTTCATGGCCACTC
AAGACAACAGCGTTAGTGTGGAAGATGTCTCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11