Sequences from confirmation amplicon


Confirmation sequences for line 869F09 and hit At1g73670 are shown.
Line ID 869F09
Locus At1g73670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27701060
Primers used for PCR 11md - 8474, show primer details
   
Sequence Name 72-K114351-0022-869-F09C-11md
GenBank Accession LN512771 [GenBank]
Sequencing primer gene specific primer, 11md: 5'-ATTCAAGAAGTAGTTGGCAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K114351-0022-869-F09C-11md
CTCACACTGGAGAAGAGTTGCTATCAAAAAGATTAACGATGTGTTTGATCATATCTCTGA
TGCAACCAGGATTCTTAGGGAGATTAAGTTGTTACGCTTGCTTCTTCATCCAGATGTTGT
TGAGATTAAACATATTATGCTTCCTCCTTCTCGAAGAGAGTTTCGTGATGTTTATGTTGT
CTTTGAGTTGATGGAATCTGATCTTCATCAGGTTATTAAAGCTAATGATGATTTAACTCC
TGAGCATCATCAGTTTTTCTTGTATCAGCTTCTTCGTGGTTTGAAGTATGTTCATGCTGG
TTAGTTTCTAAACAGTCTCTTTCACTTGTGTTATCATTATGATATAAGGTTATTGAAGAG
CTACGTCTCTGTTTGTTTGCAGCGAATGTGTTTCATCGGGATTTGAAACCAAAGAACATT
CTTGCTAATGCTGATTGCAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATG
   
Sequence Name 24-K114350-0022-869-F09C-8409
GenBank Accession LN497091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114350-0022-869-F09C-8409
TTGCATCAGCATTAGCAGAATGTTCTTTGGTTTCAATCCCGATGAAACACATTCGCTGCA
AACAAACAGAGACGTAGCTCTTCAATAACCTTATATCATAATGATAACACAAGTGAAAGA
GACTGTTTAGAAACTAACCAGCATGAACATACTTCAAACCACGAAGAAGCTGATACAAGA
AAAACTGATGATGCTCAGGAGTTAAATCATCATTAGCTTTAATAACCTGATGAAGATCAG
ATTCCATCAACTCAAAGACAACATAAACATCACGAAACTCTCTTCGAGAAGGAGGAAGCA
TAATATGTTTAATCTCAACAACATCTGGATGAAGAAGCAAGCGTAACAACTTAATCTCCC
TAAGAATCCTGGTTGCATCAGAGATATGATCAAACACATCGTTAATCTTTTTGATAGCAA
CTCTTTCTCCAGTGTGAGTATCTATAGCAGAACCAACAACTCCNTAGCTTCCTTTGCCA

Last Updated on 06.12.2018 16:11