Sequences from confirmation amplicon


Confirmation sequences for line 870C03 and hit At1g61260 are shown.
Line ID 870C03
Locus At1g61260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22594202
Primers used for PCR 53by - 8474, show primer details
   
Sequence Name 11-K114086-0022-870-C03C-53by
GenBank Accession LN491204 [GenBank]
Sequencing primer gene specific primer, 53by: 5'-AAACTAAACCCCACGGCTAGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114086-0022-870-C03C-53by
AAATCATTCTCGCACTTCATCTCCTCATTAAACTTCTTTATAAACGCCTCAACTCGCCGA
TTCAACTCTTCCTGACTCAGCGACGGCTCTTTCCTCACCTTCGCCGTCTCCGCCAACTGA
TAATAATTAGTCCTGTCCCTAAACGTATCCGACTTCTTATAAACCGGTTTCACCTCACCG
TCAACTCCCCCGGAATCTCCACGTCCAAACGTGTCAGATCTCCGGTATAACTGTCTAGTC
AACGGCGTTGATTTCCCTTCCGTTATCATCTTCCACGTATTCTCCAACGTCTCGTTCTTC
TTCGGCTTCGTCACTCTCAAAGCTCTTCCACCTGAAGAAAAGTATAATGTTAGATTACAT
ATTTACCCTTTGGTCCACCGACGCGTGTTTTCGACGCCACGCCATGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 35-K114085-0022-870-C03C-8409
GenBank Accession LN501913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114085-0022-870-C03C-8409
GCCTTTACTGGCGTGGCGTCGAAACACGCGTCGGTGGACCAAAGGGTAAATATGTAATCT
AACATTATACTTTTCTTCAGGTGGAAGAGCTTTGAGAGTGACGAAGCCGAAGAAGAACGA
GACGTTGGAGAATACGTGGAAGATGATAACGGAAGGGAAATCAACGCCGTTGACTAGACA
GTTATACCGGAGATCTGACACGTTTGGACGTGGAGATTCCGGGGGAGTTGACGGTGAGGT
GAAACCGGTTTATAAGAAGTCGGATACGTTTAGGGACAGGACTAATTATTATCAGTTGGC
GGAGACGGCGAAGGTGAGGAAAGAGCCGTCGCTGAGTCAGGAAGAGTTGAATCGGCGAGT
TGAGGCGTTTATAAAGAAGTTTAATGAGGAGATGAAGTTGCAGAGAATGGAGTCTCTCAG
ACAGTACAAAGAGATAACTAGCCGTGGGGTTTAGTTT

Last Updated on 06.12.2018 16:11