Sequences from confirmation amplicon


Confirmation sequences for line 870C04 and hit At5g05600 are shown.
Line ID 870C04
Locus At5g05600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1674539
Primers used for PCR KC82 - 8474, show primer details
   
Sequence Name 03-K103004-0022-870-C04C-8409
GenBank Accession LN487441 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103004-0022-870-C04C-8409
GCCTTTATAGAACTCAGGTCCACAAGGCAAATCCCATGTTGAATCTCATATTTCTCCTCG
TTGATTGTTTTTCTTTTATTTCTTCCCTTAAAGAAAAATTAATGTTTTGTTATTTTTATG
ATATTACGTCTGACTTGTATATGTGTACAATCATTTATATTTTGAATTTTCAAAGGATGA
TTTGCCTTCATGCATGCAAATAACAATATTGAATTGCATAGACATTTTTTTTAACAATAG
TTTTAGTTTACAATCATTTTTTAACAACATTCAAAAAAAAAAAAAAAATTTTACAATCTT
TTTTAATTTGCCCCATCTTTAATTTTGCAAGCATTTTTCAAATAGTATATTATTATAAAT
CTCTAATTTTAACGTCTATCAATATCATTTGCACACTTAAATTGTTTATTTCCAAAGGTG
TCGCGCGTCAGGTTTAAAAAACGGGGAAAAGTTAATAGTGATCAGGGAAAAGCTGGGTTA
CAAAAAAATAAAACTTTTATAAAATTGCATAATTGTTAAACTCTAAACTTCCCATGATTT
TATAAAATATGGAATATCGTTA
   
Sequence Name 51-K103005-0022-870-C04C-KC82
GenBank Accession LN507467 [GenBank]
Sequencing primer gene specific primer, KC82: 5'-ATTAAGCACCGTTCAACGATATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103005-0022-870-C04C-KC82
TGTAACCCATCTTTACCATGATCACTATCAACTTTACCACGTTTTTCAAACATGACGCGC
GACACATTAGGAAATAAACAATTAATGTGTGCAAATGATATTGATAGACGTTAAACTCAG
AGATTTATAATAATATACTATTTGAAAATTGCTTGCAAACTAATAGATGTGACAATTTAA
AAATGATTGTAAACTTCTTTTTTTTTTTTTGAAGGTGGTTAAAAAAGGATGGTAAACAAA
AACTTTGGTTAAAAAAAAGGTCTTTCCATTTCAATATTGTTTTTTGCAGGCATGAAGGCA
AATCACCCTTTGAAATTTCAAAATATAATTGATGGTCCCCTTTTCCAAGTCAAACTTATT
TTCAAAAAATTAACAAAACTTTATTTTTTTTTTAGGGGAAAAATTAAAAAAAAACCATTC
ACCGGGGAGAATTTTGAAATTCACCAGGGGATTTGCCTTGGGGACCTGGATTTCTAATAA
AGGGTTCCATGTCCGGGAAATCTCCTGGGATAACCATTGAGTATAAGGGTCAATTTGGAG
AAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCCGCACCTTT
TTTATAAAAGGAAATTACTTTTGGTTAAACCAACAAATTACAATCCGTCTTATTTTTGGG
CAAAAGCAATAAACAAATTATTCTAATTCGGAATTTTTTTTTTCGACTTGT

Last Updated on 06.12.2018 16:11