Sequences from confirmation amplicon


Confirmation sequences for line 871A05 and hit At5g45810 are shown.
Line ID 871A05
Locus At5g45810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18585262
Primers used for PCR NF63 - 8474, show primer details
   
Sequence Name 33-K102514-0022-871-A05C-8409
GenBank Accession LN500947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102514-0022-871-A05C-8409
TTACATTGAATATATTCCATTACGAAATAGATCTTAGATTTCGTCGCCATGACTTCGAAT
AGCTGAACGATGTTAGGATGACGAACACGGCGCAAGATCGAGATCTCGCGTTTGATGTGT
GCGATTAAACCGGATTTGAGAACTTTCTCTTTGTCAATTACCTTGATCGCTACGCTTTCT
CCAGATTGAGCGTTTCGTGCGAGATAGACTTTAGCGAAGGTTCCGTGGCCAAGAAGCCTA
CCCATTTCGTATTTGCCAAGGATCAGAGCTTGATGATTGCTCTGATCCTGCTTCTTCTTT
ATCGATTTCACTTTTCTTAACAAATCCGCCATTGAAAGCGAGAGATTCTCATGAAATGGT
GAAGAAAAGTTTGGATTTTGAGATCAAGATCTTCTTGATCGAAGATCGAATTCGCAAAAA
AAAAAAAAACCCTTTTTTTTTTTTGATTATGGAGGGGTTGTAAAAAAAAAAAATCTCTCT
CTCTCTCTCTCTTTTTCTGGGTTTTCCCCGCCTCCCCCGGTTCGGTTTTGGGGGTTTTTC
CCAAAAAAAGGATTTTTCTGGGGGTATTTTGGAAAATGTTCCAAATTGGGTAAACAAAAA
ATTCTCTTTTTTTGTACCCCCCCACCCCTTTTATTTCCCCTATGGGCCACCTGTTTTTTT
TTTTTTGGTTAAAAGGGCAAAAAAAACAAATCTTTTTTAAAAAATTTTTTTTTTTCCAAA
ACAACCCAAAAAAATTTTTTTT
   
Sequence Name 52-K102515-0022-871-A05C-NF63
GenBank Accession LN507775 [GenBank]
Sequencing primer gene specific primer, NF63: 5'-AAAAATTCTCCTTCGGTTTCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102515-0022-871-A05C-NF63
CATCAATTTTTCTAACATAGTATTAGTTTTTTAAGCATTTAAAGCAAAAAAAAAAAAAAC
ATTTGACCAATGGGCTATATATAGGGGTGGGGGGAAAACAAAAGGGGAAATTTTTTTGTA
TCCCATTTCGAAAACTTTCCCAAATATCCCACAAAAAAATCCCTTTTTCGGGAAAAGCCC
CTAAAACCAAACCAGAGGAAAACAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAT
CTTTTTCTTCAACACCCTCTAAAAAATAAAAAAAAAAAACGTGTTTTTTTTTTTTTGGAA
ATTCCCCCCTCCAAAAAAAAAATTTTGGTCCAAAAACCCAAATTTTTTTCCCCCTTTTTA
AAAAAACCCCCCCCTTTCAGGGGGGGATTTTTTAAAAAAAGGGAAACCCAAAAAAAAAAA
GGGGGGTAAAAAAATTTCTCCGCCTTTTATCCTGGGAAAAAAAAAAAGGGGGGGGTTTTT
TGGCCCAAAAACCTTCCCTAAATTTTTTTCCCCAAAAAACCCCTATTTGGGAAAAACCCT
CCCCCCCCGGGGTTTTTAAAAAAAAAAATTTCCCAAACCCGGTTTAACCCCCCCACTAAA
ACCGAAAATTCTTTTTTTGCCCCGGTGTTCCCCCCCAAACCCTTTTTCCTTTTTCAAAAT
CGGGGGCAAAAAATCAAAAAATTTTTTTGGAAGGGAAAAATTTCCTTTTAAAAGGGCTTC
CGGGCCGGGAAACCC

Last Updated on 06.12.2018 16:11